Study On Proliferation And Activation Of CD4 + T Cell Subsets In Peripheral Blood Of Patients With Hemodialysis Syndrome

Background Psoriasis is a kind of chronic recurrent inflammatory skin disease which cannot be completely cured. Its pathological charactoristics are keratinocyte cell higher proliferation and abnormal inflammatory cell infiltration. And its etiology and pathogenesis is not clear, Traditional Chinese medicine means that the main pathogenesis of psoriasis is "Blood heat".Much research Show that psoriasis is a kind of immune inflammatory disease with polygenic inheritance background. Thl cell was involved in the pathogenesis of psoriasis in the past years. In recent years,it was found that Th17 cell had close relationship with the pathogenesis of psoriasis.Objective In order to study the relationship between the activation and proliferation levels of different subgroup CD4+T cells and those cells in their different activation stages and the pathogenesis of psoriasis vulgaris with blood-heat syndrome, the project observed the percentage of different CD4+T cell subgroups and those cells in their different activation stages and analyzed the levels of activation related factors CD38 and HLA-DR and proliferation related factors Ki-67 of those cells.Methods Percentage of CD4+T cell subgroups and those cells in their different activation stages and the activation and proliferation levels of these cells in Peripheral blood (PB) of psoriatic patients with blood-heat syndrome and control groups were dectected by 15 Polychromatic Flow Cytometry.Results 1.(1)In PB of patients with psoriasis vulgaris of blood-heat syndrome, the percentage of effector CD4+T cells had no significant difference compared to the control group(P> 0.05), the percentage of effector CD4+T cells expressed Ki67+, CD38+HLA-DR+,CD38+HLA-DR-,CD38-HLA-DR+had no significant difference compared to the control group(P>0.05).(2) In PB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of CLA+effector CD4+T cells had no significant difference compared to the control group(P>0.05), the percentage of CLA+effector CD4+T cells expressed Ki67+was higher than the control group(P<0.05), the percentage of CLA+effector CD4+T cells expressed CD38+HLA-DR+, CD38+HLA-DR-and CD38-HLA-DR+had no significant difference compared to the control group(P> 0.05).(3) In PB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of CLA+effector Th17 cells had no significant difference compared to the control group(P> 0.05).2. (1) In PB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of central memory CD4+T cells had no significant difference compared to the control group(P> 0.05),the percentage of central memory CD4+T cells expressed Ki67+, CD38+HLA-DR+, CD38+HLA-DR-, CD38-HLA-DR+ had no significant difference compared to the control group (P> 0.05).(2) In PB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of CLA+central memory CD4+T cells had no significant difference compared to the control group(P>0.05),the percentage of CLA+central memory CD4+T cells expressed CD38+HLA-DR-was higher than the control group, (P< 0.05),the percentage of CLA+central memory CD4+T cells expressed Ki67+, CD38+HLA-DR+, CD38-HLA-DR+had no significant difference compared to the control group(P> 0.05).(3) In PB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of CLA+central memory Thl7 cells had no significant difference compared to the control group (P> 0.05),the percentage of CLA+central memory Th17 cells expressed CD38+HLA-DR+was higher than the control group, (P< 0.05),the percentage of CLA+central memory Th17 cells expressed Ki67+、 CD38+HLA-DR-、CD38-HLA-DR+had no significant difference when compared to the control group (P> 0.05).3. (1) InPB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of effector memory CD4+T cells had no significant difference compared to the control group(P> 0.05),the percentage of effector memory CD4+T cells expressed Ki67+、CD38+HLA-DR+、CD38+HLA-DR-、CD38-HLA-DR+ had no significant difference compared to the control group(P> 0.05).(2) InPB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of CLA+effector memory CD4+T cells had no significant difference compared to the control group(P> 0.05),the percentage of CLA+effector memory CD4+T cells expressed Ki67+、CD38+HLA-DR+、CD38+HLA-DR-CD38-HLA-DR+had no significant difference compared to the control group (P> 0.05).(3) In PB of patients with psoriasis vulgaris of blood-heat syndrome,the percentage of CLA+effector memory Th17 cells had no significant difference compared to the control group(P>0.05),the percentage of CLA+effector memory Thl7 cells expressed Ki67+、CD38+HLA-DR+、C D38+HLA-DR-CD38-HLA-DR+had no significant difference compared to the control group(P> 0.05).Conclusion The chronic recurrent course of psoriasis vulgaris with blood-heat syndrome could be related to CLA+central memory CD4+Thl7 cells, and the etiology and pathogenesis of "Blood-heat" syndrome could be closely related to those cytokines released by over-activated Th17 cells such as IL-17.