| Purpose： observe the effect of exogenous cold， endogenous cold stimulationon rat gastric mucosa. In the experiments give cold stimulation in ratsobservation rat gastric mucosa local pathological changes Detection of serumand gastric mucosa of gastric dynamic element，the laboratory， prostaglandins，and the changes of endothelin To investigate the effects of exogenous cold，endogenous coldpathogen on gastric mucosal function of the rat， The preventiveeffect of Liangfu pills, proves in exogenous cold，endogenous colddamage in ratgastric mucosal stimulation.Materials and methods：1The group made mould and intervention therapy.Adaptive fed the rats for3daysto adapt to the laboratorye environment. According to the weight which meetthe conditions，rats were randomLy divided into five groups：the normal groups.themodel groups：with normal saline2mL/l00g，two times a day. Cold water immersiongroups：0℃water immersion in rats30min. Water depth is2cm. Intragastric icewater groups： gastric perfusion with2mL/100g in0℃water.Composite stimulation group： In0℃water immersion in rats30min， depth of2cm， at the same time and intragastric administration with2mL/100g in0℃water.the Chinese traditional medicine group：the method with the model group，while giving the Liangfu pills orally. gastric lavage time interval of4hours.The experiment lasted7days.2Observation of the general statust.During the experiment， diet，toilet case，mental status after processing，the change in color，weight deaths and so onwere observed in rats。3The experimental indicators drawn and detection After the experiment，each experimental group rats were fed nothing but waterfor12hours. anesthesia，cut the rat’s abdominal cavity. lookde for theabdominal aorta quickly and took blood4mL canal， Waiting for measuring motilin，gastrin， prostanoids， endothelin； Cut along the greater curvature side ofthe rat stomach， saline flushing.Take gastric tissue to measure of motilin，gastrin， prostaglandin， endothelin and mucosal pathologyResult：1exogenous cold， endogenous cold evil can cause gastric mucosa pathologicalinjury in rats2Motilin, gastrin, prostaglandin level in gastric tissue in rats decreased,increased levels of endothelin.Serum levels of motilin, gastrin, prostaglandinlevels decreased, increased levels of endothelin3Liangfu pill can effectively change the model group rats injury of gastricmucosa pathological morphology.4Liangfu pill can effectively change the blood in rat model of motilin， gastrin，prostanoids， endothelinConclusion：1Cold stimulation can successfully copy the model of cold evil guest stomachof rats.2Blood and gastric mucosa in rats of motilin, gastrin, prostaglandin, changesof endothelin-1. the injury effects of cold pathogen on gastric mucosa issignificant3The degree of exogenous cold， endogenous coldand compound factors on gastricmucosal injury in different,the damage degree of composite factors most heavy,exogenous cold second, endogenous coldand late. 4Liangfu pill role that cold evil on the gastric mucosa injury is an objectiveexistence... |
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