Experimental Study On Anti - Oxidation Effect Of Acupuncture Combined With Ginseng Decoction On Chronic Fatigue Model Rats

Purpose:By observing the acupuncture combined with ginseng decoction on experimental animal body GSH and GSH-PX,SOD,MDA content, revealing the acupuncture combined with ginseng decoction on chronic fatigue rat intervention mechanism of antioxidant capacity. Material and method:Five groups of experimental design. 40 seven weeks old male wistar rats were randomly divided into blank control group, model group, control group, Chinese medicine group, acupuncture group, acupuncture combined with medicine group, a total of five groups, 8 rats in each group. With the internationally recognized exhaustive swimming modeling method combined with the bound cylinder after swimming preparing the chronic fatigue model. After the establishment of the model, the acupuncture group acupunctured Xinshu, Pishu, Shenshu, for 30 min each time daily,lasted for 7 days; Chinese medicine group with 100% ginseng decoction gavage 1.5ml once a day, for 7 days; acupuncture combined with medicine group rats daily acupunctured Xinshu,Pishu, Shenshu, at the same time with 100% ginseng decoction gavage 1.5ml once a day, for 7 days. In the end of the experiment all the rats were anaesthetized with 10% chloral hydrate, then suck out blood from abdominal aorta to keep frozen. Determination of GSH、GSH-PX、SOD、MDAcontent by ELISA method. Results: 1.Acupuncture group, medicine group and acupuncture combined with ginseng decoction can improve the chronic fatigue general symptom of rats. While the last one worked best. 2. Compared with the blank group, the model group showed that GSH and gsh-px and SOD activity decreased, while MDA content increased(P < 0.05). 3. Compared with model group, Chinese medicine group, acupuncture group these three ones, acupuncture combined with medicine group showed that GSH and gsh-px and SOD activity increased in different levels, while MDA content decreased(P < 0.05). Compared with Chinese medicine group and acupuncture group, acupuncture combined with medicine group showed that GSH, gsh-px and SOD activity increased significantly, while the MDA content decreased(P < 0.05). Conclusion: 1.Acupuncture combined with ginseng decoction can improve the antioxidant ability of rats. 2. Acupuncture combined with ginseng decoction can obviously improve the body GSH, GSH-PX, SOD activity, and decreased the content of MDA. This showed that the antioxidant capacity of fatigue rats increased. 3.Acupuncture combined with ginseng decoction on antioxidant capacity of fatigue rats was obviously higher than that of acupuncture alone or medicine alone.