The Early Effects Of Hydrogen Peroxide On The Mucosa Of Human Fallopian Tube-A Morphological And Stereological Study

Purpose: The early effects of hydrogen peroxide on the morphological features of human fallopian tubal epithelium were investigated by light, transmission and scanning electron microscopy. Additionally, stereological technology was used in order to study the safety of hysterosalpingography with hydrogen peroxide solution. Methods: Thirty patients with uterine myoma or adenomyoma were randomly divided into two groups (control group with saline injection and experiment group with hydrogen peroxide solution injection). Their fallopian tube and endometrium were obtained at salpingohysterectomy. The histology and ultrastructure of fallopian tube were observed by light, transmission and scanning electron microscopy. Meanwhile, the mitochondria parameters were analysed by stereology. Results: 1.Light microscopy Comparing with control group, the experiment group displayed the following morphological features: the tubal plicaes were irregular and the mucosa were edematous and congested. In plical stroma, inflammation cells infiltrated. In some place, the epithelum was separated from surface. All these changes were most pronounced 2 hours after hydrogen peroxide injected. After 24 hours, congestion and edema were still present in mucosa and submucosa, but with less degree intensity. 2.Scanning electron microscopy The ultrastructure of tubal mucosa changed obviously in experiment group. The cilliae were disorderly arranged or adhered to each other, some of them were dissociated from ciliated cells. Two hours after hydrogen peroxide injection, the secretory cells were deformed and showed a fold- or hollow-like appearance. All these changes remained even after 24 hours. 3. Transmission electron microscopy In the experiment group, a distinct dilatation of mitochondria and rough endoplasmic reticulum(rER) were visible both in ciliated cells and secretory cells. The cristae of mitochondria decreased. The heterochromatin was aggregated marginally. Some cilliae of ciliated cells and microvilla of secrectory cells were dissociated from cell surface. 4. Stereological study In comparing with control group, the volume density and mean volume of mitochondria in ciliated and secretory cells increased obviously, and among experiment groups, the differences were most significant 2 hours after hydrogen peroxide solution injection, but the numerical density showed no significant change. Conclusions: From the above mentioned results, we concluded that hydrogen peroxide can affect the morphology and ultrastructure of human fallopian tubal epithelium. Additionally, it cause an increase in the volume density and mean volume of mitochondria, but has no significant influence on the numerical density.