CHINICAL STUDY ON RELATIONSHIP OF TCM DIFERENTIATION IN CHRONIC RENAL FAILURE WITH THE CHANGE OF PLASMA ENDOTHELIN,NITRIC OXIDE AND 6-KETO-PGF_1α

Chinical Study on Relationship of TCM Differentiation in Chronic Renal Failure with the Change of Plasma Endothelin, Nitric Oxide and6-keto-PGF,aMajor: One of the fafcgafed Tradttxiai Chinese and VVt-sfcm Merifcne Postgraduate: Li Zhuangmiao Tutor: Prof. Du JianABSTRACTBackground: The imbalance of synthesis and releasing of endothelin (ET), nitric oxide (NO) and prostacyclin (PGI2), endothelium-derived relaxing and contracting factors, has been implicated in the pathogenesis of chronic renal failure (CRF).Objectives: To investigate the change of ET, NO and PG12 in patients with CRF. To discuss the relationship between syndrome differentiation of TCM and the change of ET, NO and PGI2 in patients with CRF.Subjects and Methods: 50 patients (34 male and 16 female) with CRF, the average age was 51.82 ?13.15 years (aged 25-70 years), were observed, among them 11 cases in group of the renal compensation and decompensation stages, 39 cases in group of the renal failure and uremia stages. Of the 50 patients, 32 cases were hemodialysis, 18 cases were non-hemodialysis, and 30 cases in group with hypertension complication, 20 cases with non-hypertension complication. Through the TCM differentiation, the 50 patients included 21 with the deficiency syndrome complicated with excess symptoms, 29 with the excess syndrome complicated with deficient symptoms. At the same time, 30 healthy people (21 male and 9 female), aged 28-67 years were taken as the control group. To determine the plasma endothelin and 6-keto-PGF, ?by RIA method, the serum nitric oxide by reductase nitrate colorimeteric methodResults: I. The level of ET was significantly increased (PO.01), but the levels of NO, NO/ET and 6-keto-PGF, ?significantly lowered in patients with CRF compared with the control group (PO.01); 2. The level of ET was increased with the deteriorating of renal function (P<0.01), among them the group of renal compesation and decompesation stages was significantly different with the group of renal failure and uremia stages (PO.01); the level of No was lowered with the deteriorating of renal function, among them the group of renal compesation and decompesation stages was obviously different with the group of renal failure and uremia stages (PO.01); the levelof NO/ET was lowered with the deteriorating of renal function {PO.OI), among them the two groups were significantly different (PO.05); the level of 6-keto-PGF, ?was also decreased with the deteriorating of renal function, but the two groups had no obvious difference. 3. The levels of ET and NO were significantly decreased after the 32 patients had been hemodialysed (PO.O I and PO.Q5), but the levels of NO/ET and 6-keto-PGF, ?were not obviously changed 4. The level of ET in the group with hypertension complication was significantly higher than the group with non-hypertension complication (PO.OI), but the levels of NO and NO/ET were obviously lowerer than the group with non-hypertension complication (PO.OI). 5. The amount of the group of deficiency syndrome complicated with excess symptoms was significantly different with the group of excess syndrome complicated with deficient symptoms on the various stages of renal function. The deficiency syndrome complicated with excess symptoms showed frenquently in the stages of the rentd compesation and decompesation, but the other showed frequently in the stages of the renal feflure and uremia. 6. The levels of ET, NO/ET in the group of deficiency syndrome complicated with excess symptoms were significantly different with the control group (P