STUDIES ON THE ACTIVE CONSTITUENTS OF Ervatamia Yunnanensis Tsiang

Dried stem of Ervatamia yunnanensis Tsiang is a folk medicine used for the treatment of hypertension and abdominal pain. The plant is a shrub or arbor distributed in the Yunnan and Guangxi provinces. Total Ervatamia yunnanensis Tsiang alkaloid showed significant antinociceptive effects and cerebral protecttive effects. In order to find the active constituents, fifteen compounds and a mixture with two alkaloids have been isolated from the total Ervatamia yunnanensis Tsiang alkaloid and the other part. The structure of the compounds were elucitated by the analysis of EI-MS, 1H-NMR, 13CNMR and 2D-NMR. In the pharmacological experiments, the total Ervatamia yunnanensis Tsiang alkaloid showed the effects on block of rein~tatement of place preference in relapse model morphine dependment mice. And it didn't show the psychic dependence and physical dependence itself in mice. The compounds are showed as the following:SUB 1f3-sitosterolSUB 2dancosterolSUB 3syringaresinolSUB 4pinoresinolSUB 5lupenyl acetateSUB 6matrineSUB 7coronaridineSUB 819, 20-dihydroisostitsirikineSUB 91 9-s-heyneanineSUB 10ibogamineSUB 11EYA-lSUB 121 9-s~voacangarine and 11 -methoxyl- 1 9s-heyneanineSUB 13EYA-2SUB 14conodurineSUB 1519, 20-E-vallesamineSUB 1610-hydroxy-19, 20-dihydroisositsirikineSUB2~ SUBS. SUB8~ SUB9~. SUB12-. SUB13.. SUB14 and SUB 15 were isolated from Ervatamia yunnanensis for the first time. SUB3 .. SUB4 -. SUB6 were first isolated from Ervatamia genus. SUB 11 and SUB 16 are new compounds.