Experimental Study Of Rapid Detection And Identification Of Pathogenic Fungal Species Inducing Deep Fungal Infection By Polymerase Chain Reaction Assay

Recently, the incidence of deep fungal infection stepped up increasingly followed by the widely using of antibiotics, corticosteroid hormone and immunosuppressive agents, moreover, the mortality reach to 30%-60%. It was also found much fungal species with drug resistance. Therefore early diagnosis was urgently required for helping to treat deep fungal infection clinically.The aim of this study is to aim to establish a kind of PCR assay that could detect and identify the species of pathogenic fungi inducing deep fungal infection accurately, specifically, rapidly and sensitively. The first part was to establish a PCR system that could detect fungi rapidly, and use the species-specific primers ITS4, ITS86, combined with genescan analysis technique, to identify 23 species of pathogenic fungi inducing deep fungal infection, thus establishing the method of detection and identification. The second part was to detect and identify the species of pathogenic fungi suspended in vitro in bleed, urine, pulmonary washes cerebrospinal fluids with the new established method. The third part was to detect and identify the fungal species in dynamic blood specimens of a rabbit animal model of disseminated Candidiasis in different immune status with the new established method. Through the experiment in vivo and vitro, it was confirmed that the rapid PCR assay we established which could detect and identify the species of pathogenic fungi inducing deep fungal infection really was an accurate, specific, rapid and sensitive method for early diagnosing deep fungal infection.