The Experimental Study On The Effects Of The Vacuum-Assited Closure Technique On The Microcirculation Of The Wound

In this experimental study, an acute full-thickness wound on the rabbit ears was established and used to explore the dynamic changes in the microcirculation of the wound treated with the Vacuum-Assisted Closure (V.A.C.) technique. The study includes five parts: 1. The changes of blood flow of the wound after applying V.A.C. was measured by the Laser Doppler Flowmeter (LDF); 2. The changes of blood velocity and capillary diameters of the wound after applying V.A.C. was observed and measured by the microscope and computer-assisted microcirculatory analysis system; 3. The wound healing process of both the V.A.C.-treated group and the control group was observed. The rate of wound healing was determined by image analysis system. Use the H.E. method to explore the migration and proliferation of epithelial cells, granulation tissue formation, the amount of wound fluid, etc; 4. The functional capillary density of the wound being treated with V.A.C. was measured by the microscope and computer-assisted microcirculatory analysis system and compared to the controls; 5. The changes of morphology and structure of capillaries, vascular endothelial cells (VEC), capillary ectoblast and VEC clefts , etc, of the wound being treated with V.A.C. was observed by electronic microscope and compared to the controls. Results: 1. The local blood flow significantly (P