Effects Of Propofol On NOS And NO Change Of Different Anesthesia Phase Rat Brain And On Membrane Fluidity And [Ca～(2+)]_i Of PC12 Cells

Part I Effects of propofol on NO and NOS change of different anesthesia phase rat brainObjective To investigate effects of propofol on NO products and NOS activity change of different anesthesia phase rat brain,and discuss its relevant mechanism.Methods fifty rats were divided randomly into five groups.The animals were injected introperitoneally(ip) propofol 100mg kg-1 or normal saline 10ml ?kg-1(control group)respectively.There rats were immediately decapitated before(induction group) and after(anesthesia group) having disappeared righting reflex,and when righting reflex appeared again(recovery group),and one hour appeared again(awake group).NO production and NOS activity were estimated by spectrophotometer.Results After ip propofol 100mg ?kg-1,rats righting reflex disappeared and NOS activity and NO production of cortex,hippocampaL,brain stem,cerebellurm were inhibited significantly(p<0:01).Following rats righting reflex appeared again, NOS activity and NO production of cortex,hippocampal,brain stem\cerebellurm rise again.Conclution The changes of .NO production and NOS activity are accorded with behavior change of rats. As secondly messager,NO of rat brainmay be related the central inhibitory effects of propofol.