The Expression Of Mannose-ligand Receptor(MR) Of Human Sperm And The Relationship Between MR Expression And Sperm-zona Pellucida Interaction

Objective:1.To investigate the relationship between mannose-ligand receptor (MR) expression and the physiologic status of the sperms;2.To identify the effect of MR on sperm-zona pellucida interaction.Methods:1. After being incubated with hFF, sperms were labelled with a fluoresceinated a -D-Mannosylated- FITC-albumin (FITC-DMA) to detect the MR expression. At the meanwhile, sperms were assessed by CTC assay to determine their capacitation and acrosome status. Then correlation between the expression of MR and the percentage of thephysiologic status of the sperm was analysed.2. Preincubating sperm with DMA to study the effect of DMA on sperm binding to the zona in the hemizona assay (HZA). At the same time, the spermatozoa were labelled with FITC-DMA to detect the relationship between the expression of MR and the number of the sperm binding to the hemizona.3.In order to detect the effect of MR on the sperm-zona binding deeply, DMA was "introduced in the medium after the sperm binding to the hemizona.Results: l.hFF enhanced capacition and induced AR of sperm, meanwhile it improved the expression of MR;2.The expression of MR was not associated with capacition status monitored by CTC assay (P>0.05) but was correlated to AR (PO.05);3.DMA did not effect the binding of sperm to the hemizona , but it could made the sperm that have bound to the zona during a 4-hour period detach from zona;4.The expression of MR was not associated with the number of the sperm binding to the hemizona.Conclusions: l.hFF can induce AR of sperm, meanwhile it can alsoimprove the expression of MR;2.Expression of MR is rather the result of AR than that the essence of capacitation;3.MR is not necessary for the primary recognition between sperm and zona, it may be related to the second recognition and binding.