The Experiment Study Of Liu-Shen-Wan On Inducing Apoptosis In HL-60 Cells

Purposel.To explore the mechanism of liu-shen-wan in treating AML from inducing apoptosis and to supply the theorical basis in clinical therapy.2.To enlarge the clinical application of liu-shen-wan. MethodI.Chinese herbs serum pharmacology method produces medicine serum of Liu-Shen-Wan2.Sha shi distillation method extracts Liu-Shen-Wan, with alcohol as the solvent.3.MTT assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells.4.GIMSA dying assay observes morphology changes of apoptosis.5.In situ end-labeling method (POD) probes apoptosis phenomena Resultsl.MTT assay indicates:Liu-Shen-Wan alcohol extraction has obvious growth inhibition effect on HL-60 cells, and has dependency on concentration. ICso is 0.5ug/ml. As far as the growth inhibition effect is concerned, the middle-dose group of Liu-Shen-Wan medicine-serum is superior to the low-dose group and high-dose group.2.GIMSA dying assay exhibits: HI-60 cells dealed with Liu-Shen-wan alcohol extraction and Liu-Shen-Wan medicine-serum presents typical apoptosis morphology, such as cell membrane shrinkage, chromatin contraction and rupture, apoptosis body formation.3.In situ end-labeling method (POD) indicates:HL-60 cells apoptosis induced by Liu-Shen-Wan alcohol extraction have time-concentration dependency relation within certain ranges; The HL-60 cells apoptosis effect induced by Liu-Shen-Wan medicine-serum is superior to AS2O3 serum group and blank serum group.(P<0.01)ConclusionsLin-shen-wan can induce apoptosis in HL-60 cells in vito.Inducing apoptosis is one of possible mechanisms in treating AL by Liu-Shen-Wan.