Expression And Significance Of PSA And PCNA In Prostatic Carcinoma And Prostatic Hyperplasia

Objective To study the patterns of the expression of PSA andPCNA in prostatic carcinoma and prostatic hyperplasia and toevaluate their relationships to the biological malignant potentials of prostatic carcinoma.Methods The expression of PSA and PCNA in 44 cases of formalin-fixed, paraffin-embedded prostatic carcinoma tissues, and 20 case of prostatic hyperplasia tissues was examined using antibody of human PSA and PCNA, and Streptavidin-HRP(SP) immunohistochemical method Was adopted. The results were analysised statistically.Result PSA was expressed diffusely in the cytoplasm and partly in the nuclei of the cancer cells.PSA was found in 23(52.27%) of 44 prostatic carcinoma samples, 16(80%) of 20 prostatic hyperplasia tissues . the positive rate of PSA in prostatic hyperplasia tissues was significantly higher than that in the prostatic carcinoma tissues. PCNA was expressed diffusely in the nuclei of the cells, the positive rate of PCNA in prostatic carcinoma tissues was significantly higher than that in the prostatic hyperplasia tissues. The expression of PCNA was different with different Gleason scores.Conclusion The expression of PSA was correlated inversely with Gleason scores and clinic grades, no significant correlation was observed between the expression of PSA and the PSA lever in sera. The expression of PCNA was correlated with Gleason scores, no significant correlation was found between the expression of PCNA and clinic grades .In short PSA and PCNA are useful markers for detecting prostatic carcinoma and predicting recurrence of prostatic carcinoma.