| Objective:The article aims:1. To study the effects of haloperidol(Hal) on electrophysiologic parameters in vivo rabbit heart.2. To study the effects of haloperidol on electrophysiologic parameters in isolated rabbit heart, eliminating action of nerves and body fluid by using standard Langendorff technique.3. To study the effects of haloperidol on experimental arrhythmias by using various models of experimental arrhythmia.Material and method:1. The effects of haloperidol on electrophysiologic parameters in vivo rabbitheart.The rabbit was anaesthetized with 3% sodium pentobarbital(30mg 'kg'1) by intravenous infusion. The arterial pressures were monitored with a catheter put into the ascending aorta through left common carotid artery. One 5F Cordis electrode catheter of four electrodes was inserted into right atrium through the right external jugular vein. The thorax was opened via thoracotomy along the left edge of the sternum. Maintain autonomous breathing and sinus rhythm. A ventricular plunge electrode of four electrodes was inserted into the left ventricle under the left auricle and beside the anterior interventricular sulcus. The third electrode and the fourth electrode were connected with SEC-2102 model stimulator. The first electrode and the second electrode were linked to RM-6000 model polygraph physiological recorder and FC-14 model tape recorder. The ECG-6511 model electrocardiogram recorder was connected with the needle electrodes pierced into the extremities of rabbit to record the limb lead electrocardiogram. In every group, at lOmin pre-drug and at 10, 30, and 60min after NSlOml orhaloperidol l.Omg ?kg' or 2.0mg ?kg" iv, the effects of haloperidol on arterial blood pressure and cardiac electrophysiologic parameters were determined respectively, which were systolic arterial blood pressure(SABP), diastolic arterial blood pressure(DABP), mean arterial blood pressure(MABP), atrial diastolic threshold(ADT), atrial relative refractory period(ARRP), atrial effective refractory period(AERP), ventricular diastolic threshold(VDT), ventricular relative refractory period (VRRP), ventricular effective refractory period (VERP), heart rate(HR), sinoatrial conduction time(SACT), sinoatrial node recovery time(SNRT), Wenckebach's cycle length(WCL) and 2:1 blocking point(2:lB).2. The effects of haloperidol on electrophysiologic parameters in isolated perfused rabbit heart.In the isolated rabbit heart perfused by using standard Langendorff technique, two plunged electrode of four electrode were respectively inserted into the right atrium and the left ventricle under the left auricle and beside the anterior interventricular sulcus. The third electrode and the fourth electrode were connected with SEC-2102 model stimulator. The first electrode and the second electrode were linked to RM-6000 model polygraph physiological recorder and FC-14 model tape recorder. In every group, at lOmin perfused by Krebs-Henseleit(K-H) perfusion liquid and at 10, 25 and 45min perfused by K-H perfusion liquid with haloperidol O.OOumol ?L"1 or 0.75u.mol ?L"1 or l.SOumol ?L"1, the effects of haloperidol on its electrophysiologic parameters were observed respectively, which were ADT, ARRP, AERP, VDT, VRRP,VERP, HR, SACT, SNRT.3. The effects of haloperidol on experimental arrhythmias.The effects of haloperidol on experimental arrhythmias induced by chloroform, Cacl2, chloroform-adrenaline, cedilanid and electrical stimulation in various animals were observed. The mice hearts were exposed immediately via thoracotomy when their respirations were inhibited by inbreathingchloroform and the incidences of ventricular fibrillation were observed. After anaesthetized by urethan(1.2g ?kg"1, ip), ventricular fibrillations were induced in Wistar rats with 3.5%Cacl2(140mg ?kg"1) by intravenous infusion and the incidences of ventricular fibrillation were observed. Arrhythmias were induced with 0.01% adrenaline(50ug ?kg"1) by intravenous infusion in rabbits anaesthetized by inhalation of chlorof... |
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