Expression Of Testicular EGF In Varicocele Rats And Influence Of Shengjing-Soluble-Granule To EGF Expression

Objective: To prove EGF expression in varicocele rats testis.To discuss influence of Sengjing-soluable-granules to testicular EGF expression in varicocele rats.Then discuss and reveal molecular mechanism of varicocele sterility from molecular level MethodsiExperimental varicocele was created in male rats by partial ligation of left renal vein .The study were divided into 3 groups randomly: varicocele group(VG), sham operation group (SOG),treatment group(varicocele+chinese traditional medicine group VMG).The histology was observed at the 3rd postoperative month .Resultsrlt was found that (1) the left testicular weight in VG was far lower than that in SOG(P<0.05).(2) the pathologic changes in VG were severer than that in VMG .(3)Amount of EGF positive leydig cell decreased in varicocele testicular tissue on the whole .(4)most of leydig cell showed EGF intensely positive in VMG rats testis.Conclusions: According to the experiment confirmed that soluble granules of Shengjing protected can protect the testicular tissues of varicocele rats and alleviate testicular pathologic damage ;The amount of EGF expression decreased in varicocele rats testis.