The Influence Of Single Chinese Medical Herb On The Movement Of Dog Gallbladder

Cholelithiasis is the most common disease in our country. The effect of most Chinese medicine prescription is different. The main reason is that the composition of single Chinese medical herb in prescription is different. Cholecystokinin ( CCK) is one kind of polypep-tide secreted by intestinal mucosa which can make gallbladder contract. We selected five kinds of Chinese herbs from different choleretic prescriptions, let dogs take every kind of herb decoction, measured the size of gallbladder before and after taking medicine by means of B ultrasound, determined serum CCK concentration before and after drug taken. The effect of every kind of Chinese herb to gallbladder movement and mechanism were discussed.Experimental Materials1. Ten hybrid dogs, 10.5 - 15 kg in weight.2. Chinese medicine: 250g of Herba Lysimachiae, 100g of Radix Aucklandiae, 150g of Radix Curcumae, 150g of Radix Et Rhizoma Rhei, 150g of Fructus Aurantii, supplied by our hospital. The concentration were herba Desmodill 1. 0/ml, Radix Aucklandiae 0.5/ml, Radix Curcumae 1. 0/ml, Radix Et Rhizoma Rhei 1. 0/ml, FructusAurantii 1. 0/ml. These drug were sealed for later use.3. Aloka SSD - 210DX ultrasound machine, the frequency of probe was 3. 5 MHz. HITACHI 05PR -22 hypothermic centrifugal machine. Cholecystokinin radio immune reagent kit.Experimental Methods1. Method: Ten dogs were numbered. The interval between the second experiment from the first was seven days. Fasting twelve hours before experiment .1) Anesthesia: by abdominal cavity injection of 2. 5% sodium pentothal.2) Fixed dogs on platform, intubated stomach tube from oral cavity. Blood was taken before, fifteen, thirty, sixty and ninety minutes after drug injection from tubes meanwhile the size of were measured.3) Blood taken and preservation after centrifugation: 5ml blood were taken from vein in right upper arm, then blood was added in pre-cold test tube treated with anticoagulant and trasylol. Plasma was preserved under negative twenty degree centigrade after hypothermic centrifugation.4) Gallbladder measurement; Set probe on right costal margin, measure maximal longitudinal diameter, cross section diameter and, gallbladder volume was calculated by following formula: V = ( L x Wx H ) x /6 ( V: gallbladder volume, L: maximal longitudinal diameter, W; maximal cross section diameter, H: maximal cross section height, π=3.14)5) CCK value were determined in all plasma by radioimmunal assay.2. Data treatment; Every parameter was showed as average mean ?standard difference. Average mean comparison among groups adopted pair t - test. Correlation analysis was applied between gallbladder size and CCK.Experimental Results1. The volume of gallbladder reduce remarkably thirty minutes later after perfusion of Herba Lysimachiae and Radix Curcumae to stomach compared with that of fasting ( p < 0.01). Dog gallbladd Herba Lysimachiae er reduce the most remarkably thirty minutes after drug perfusion of Herba Lysimachiae and Radix Curcumae. The reduce degree were 38. 7 percent and 38. 4 percent respectively compared with that of fasting.2. The volume of dog gallbladder reduce remarkably thirty minutes after perfusion of Radix Aucklandiae compared with that of fasting. . Dog gallbladder reduce the most remarkably thirty minutes after drug perfusion of Radix Aucklandiae. The reduce degree was 25 percent.3. The volume of dog gallbladder had no change after Fructus Aurantii perfusion to the stomach.4. The volume of gallbladder reduce remarkably thirty minutes later after Radix Et Rhizoma Rhei perfusion to dog stomach compared with that of fasting ( p < 0. 01). But the volume enlarge remarkably ninety minutes later compared with that of fasting. The reduce and enlarge degree were 25.2 percent and 27. 6 percent respectively.5. Dog plasma CCK concentration increased remarkably thirty minutes later after Herba Lysimachiae and Radix Curcumae perfusionwith that before perfusion( p < 0. 01).6. Dog p...