| Oral lichen planus (OLP) is non-infectious mucoal disease with unclear etiology.lt is histopathologically characterized by liquefactive degeneration of basal cell and the band-like lymphocyte infiltrate in the lamina propria close to basal cell. In recent studies, T-lymphocyte-mediated cellular immunologic response was found involved in OLP. Furthermore, cellular immunologic response is intimate assioated with activation status of antign-presenting cell (APC). LC is a kind of dendritic cells(DC) distributing on epiderm and mucosa including oral capitity as well as is a prefessional APC which have large potentiality to initiate naive T cell reaction. Limited data on the role of pathological process in OLP were reported in China,and the possible roles of LC in OLP remains unknow.By means of immunohistochemical and image analysis technique, this research studied pathological mechanisms and the role of LC in OLP. Thechange of number and structure of LC in OLP were observed andoffered experimental data to studying the role of LC in OLPpathogenesis.MATERIALS AND METHODSFourty-one cases of formalin-fixed,paraffin-embedded specimens from OLP lesions were obtained from the pathological archives of the stomatological hospital of ZhengZhou city.These specimens included 16 cases of simple-form, 25 of erosive-form OLP. 7 cases of specimens from oral normal mucosa were taken as control. In the same time, Piboonniyom's clinical assessmant was used to quantitate each case lesional extent. LCs were labeled with anti-CDla ,HLA-DR monoclonal antibodies, while infiltrated T lymphocyte were labeled with CD3,CD45RO monoclonal antibodies by immunohistochemical elivision techniques. HPIAS-1000 image pattern analysis system was applied to demonstrate the mean gray degree, size and perimeter of positive LC in epithelial and lamina propria layer. All data including clinical assessmant of OLP were statistically analysed. RESULTS:1. Lesional extent of OLP quantitated by Piboonniyom's clinical assessment:The average of simple-form and erosive-form OLP were respectively 9.53?.44; 14.82?.66.There was significant difference between two forms in lesional extent.2. The structure,distribution and amount of CDla-positive LC in OLP and normal groups: the dendrite of CDla-positive LC in OLP became longer than in normal groups.The distribution of CDla-positive LC in OLP trended to surface layer of epitheial,even reaching keratinization. In epithelial CDla-positive LCs distribution was relatively uniform in epithelial and scattered in lamina propria.The immediate contact between CDla-positive LC and T lymphocyte could be observed. The number of CDla-positive LC in normal musuca, simple-form and erosive-form OLP was respectively 2.51 ?.60,5.10 ?.91 and8.42 ?3.01 and there was significant difference between each other(P<0.01). The mean gray degree of CDla-positive LC of epithelium in normal musuca, simple-form and erosive-form OLP were 190.12?4.62,148.59?0.93 and 146.20?3.26.Those of simple-form and erosive-form OLP significantly lower than in normal musuca(P<0.05). There was significant difference between the mean size and perimeter of CDla-positive LC in normal musuca and in OLP(P<0.05).3. The amount of CDla-positive LC and HLA-DR-positive LC in normal groups, simple-form and erosive-form OLP were respectively2.91?.34 and 1.89?.47, 7.60?.30 and 2.93?.05, 11.18?.34 and 4.53?.52. The number of CDla-positive LC was significant difference versus HLA-DR positive LC in OLP(p<0.01),but no significant difference between them was found in normal musuca(P>0.05). The means gray degree ofHLA-DR-positive LC in epithelial of normal musuca , simple-form and erosive-form OLP were 165.99 ?5.54, 103.05 ?1.61 and 98.67 ?6.68 respectively(low gray degree implying strong expression), and there was significant difference between normal and OLP groups (P<0.01).4. The distribution and amount of CD3-,CD45RO- positive T lymphocyte in OLP groups: CD3-,CD45RO- positive T... |
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