| Ischemia-reperfusion injury (I/R) often occurs during the pathological processes such as angiopathy, extracorporeal circulation, cardiothoracic surgery, constitution or organ transplantation and replantation surgery, etc. The mechanism of I/R is related to the changes of oxygen free radicals (OFR), calcium overload, inflammatory reaction, apoptosis, etc. which is showed by some research. In addition, I/R has injurious or/and inhibitory effects on the neoplasm tissue. Oxidative injury and apoptosis are important factors in the process of it. But only few relative research papers have been found and the detailed mechenism is not clear. How does I/R affect and/or injure the normal tissue and carcinomous tissue? Wheather can we use I/R to treat hepatic carcinoma or not? All of them are worth studing. So we have established the rabbit I/R liver carcinoma implantation animal models to explore the influence on carcinoma tissue by I/R and its pathophysiological mechanism, which can lay a basis on the establishment of new methods of healing the specialy hepatic carcinoma.ObjectiveTo explore the changes and the injurious roles of OFR by detecting andanalyzing the changes of nitric oxide (NO), nitric oxide synthase (NOS), super-oxide dismutase (SOD) and malondialdehyde (MDA) concentration of the normal hepatic tissue and the carcinoma tissue respectively after I/R, and to explore the influence and implication of carcinomous tissue following I/R by morphologic observation and quantitative analysis of apoptotic cells by hematoxylin eosin (HE) dyeing and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL).MethodsThe hepatic carcinoma animal model was established by the ultrasonography-guided implantation of VX2 carcinoma constitution mass into the left-middle hepatic lobe of the rabbits. Ischemia was caused by using a non-traumatic vascular clamp to block the branches distributing into the left-middle lobe of the hepatic artery for 60 minutes, and then the clamp was removed and the reperfusion injury of the hepatic carcinoma occurred. The animal models were divided into the control group (before I/R), and Omin, Ih, Id, 3d and 7d groups after I/R randomly, and then their normal hepatic tissues and carcinomous tissues were taken out for the respective NO, NOS, iNOS, SOD and MDA detect, for formaldehyde solution fixation, paraffin embedding, and slice subsequently, for HE dyeing, and for TUNEL. According to these data, the apoptotic conditions of the normal hepatic tissues and the carcinomous tissues were analyzed by compare and contrast.Results(1) The NO concentration of the hepatic tissue of all groups except Ih group was lower than that of the control group and changed a little. The NO concentration of the carcinomous tissue decreased after I/R and reached the lowest level at Ih and then restored gradually, but retained a lower level than thatof the control group at 7d (PO.01 ) . The difference between the NO concentration variations of the normal hepatic tissue and carcinomous tissue with time after I/R is very significant (PO.01).(2) The NOS concentration of the hepatic tissue including eNOS, nNOS and iNOS decreased a little after I/R, of which the iNOS concentration decreased apparently from Omin to 1 h ( PO.O1) and did not restore to the normal level of the control group at 7d yet. The NOS concentration of the carcinomous tissue first decreased below the normal level of the control group at Omin after I/R and then rose over it from Id to 7d (PO.01) , but the iNOS concentration rose continuously and beyond the normal level of the control group from Omin to 7d(PO.01) . The difference between the NOS concentration variations and the iNOS concentration variations of the normal hepatic tissue and the carcinomous tissue with time after I/R is very significant (PO.01).(3) The SOD concentration of the hepatic tissue decreased quickly after I/R and then restored gradually, but still retained a lower level than that of the control group at 7d (PO.01) . The trend of the SOD concen...
|
PDF Full Text Request