Influence Of Agkistrodon Acutus Snake Venom On The Invasion Of Toxoplasma Gondii Tachyzoites To Host Cells

Objective: 1 To observe the Toxoplaxma gondii tachyzoites infection of host cells in the culture system with the presence of Agkixtrodon acutus snake venom originated from the Southern Area of Anhui Province.2 To observe the influence of Agkixtrodon ccutus snake venom on time to the mini quantityof Toxaplaxm gondii tachyzoites invading to host cells.3 To explore the possible value for pathogenic diagnosis of toxoplasmosis with the snake venom as an adjuvant by method of cell culture.Methods: 1 The snake venom was added in the tachyzoile-Hela cell culture system in different quantity ,then the infected cells were counted in different time(3~12h) by light microscope with the Giemsa stain and by fluorometric microscope with IFA respectively, to observe the effect of snake venom to the Toxoplasma tachyzoiteinvasion to host cells.2 The different dosage of snake venom was added in the tachyzoite -THP-1 cell culture , to detected the infected cells by flow cytometer(FCM), and to observe the effect of different account snake venom to the tachyzoite invasion.3 The same quantity of snake venom was added in the tachyzoite-Hela cell culture with mini quantity of tachyzoites , to observe the invading time of tachyzoite to host cells.Results: 1 With the adjuvant the infecting rate of host cells was higher remarkably than that of the control group, with 5.00 u g/ml and 0.25 u g/ml of the adjuvant the infecting rate was increased 124.00% and 91.00% respectively.2 With the adjuvant the infecting rate of Hela cells was higher remarkably than that of the control group, 5.00 P g/ml and 0.25 n g/ml snake venom were added in theof Heia-tachyzoite system. With the 5.00 u g/ml and 0.25 u g/ml snake venom ,the Hela cells infecting rate was increased 159.00% and 49.00% in the 105/ml tachyzoite group respectively; 127.00% and 52.00% in the 104/ml tachyzoite group respectively; 73.00%and 20.00% in the 103/ml tachyzoite group respectively.3 The rate of THP-1 cells infected by Toxoplasma tachyzoite was higher remarkably than that of the control group, with 5.0 u g/ml and 0.25 u g/ml of the adjuvant ,the infecting rates was increased 196.50% and 38.00% respectively.4 The time of the tachyzoites appearance in the host cells was obviously decreased in the tachyzoite-culture cell with snake venom.Under the dosage of 5.0 M g/ml snakevenom the tachyzoites appeared in the host cell at 45 min with inoculation of 102/ml tachyzoites, and the matched control appeared in the host cell at 75 min; the tachyzoites appeared in the host cell at 60 min with inoculation of 101/ml tachyzoites, and the matched control appeared in the host cell at 105 min .5 The excess snake venom can cause the culture cells to death after three hours.Conclusion: The infecting rates of Hela and THP-1 cells with the presence of Agkistrodon acutus snake venom were remarkably higher than that of the control group. The dose of 5.00 g,ml was a suitable given dose . with the 5.00 g,ml snake venom the time of tachyzoite invading was shorter obviously than the contral. The excess snake venom can cause Hela cells to death, but, for the THP-1 cells hadn't this phenomenon. As an useful adjuvant the Agkistrodon acutus snake venom promoted process of invading to host cells increased the infective rate in a given dose, so we can use the snake venom as an adjuvant in the practical work to increase the positive rate and reduced the time of tachyzoites detecting .