The Lipids Modulation And LPL Gene Expression Effects Of The SSYZ Caspule On Rats With 5/6 Nephrectomy

Background and Objective: Dyslipidemia occurred in the majority of patients with chronic renal failure. The cause of dyslipidemia is multifactorial. Reduced lipoprotein lipase (LPL) activity played a key role. Dyslipidemia not only leaded patients with renal disease to belonging to a very high cardiovascular risk group but also aggravated renal sclerosis. Dyslipidemia should most likely be subjected to sufficient lipid-lowering therapy in preventing nephropathy process. The purpose of the study was to investigate the therapeutic effect of capsule of shen-shuai-yang-zhen(SSYZ) on lipid metabolism disorder and renal function in rats with 5/6 nephrectomy, Simultaneously, evaluate the cordis Lipoprotein Lipase gene expression, trying to find the therapeutic mechanism of the SSYZ to the CRF rats.Methods: 1. SD rats were made into CRF model by 5/6 nephrectomy. Rats were randomly sorted into the following groups. Normal control group(NC), CRF control group(CC), Gemfrozil group(C+G), High dose ssyz group(C+HSSYZ), Moderate dose ssyz group(C+MSSYZ), Low dose ssyz group(C+LSSYZ). All rats were treated with corresponding drugs for 4 weeks. During and after the treatment, the general state, serum albumin(AL B), total cholesterol(TC), triglyceride(TG), low density lipoprotein cholest er-ol(LDLc), very low density lipoprotein cholesterol(VLDLc) and high density lipoprotein cholesterol(HDLc) were detected.2. Blood urea nitrogen contents(BUN) and serum creatinine(SCr) were detected. The kidenys of the rats were used for the study of nephron pathology. Kidney pathological features were observed under microscope.3. The myocaridum of the rats were used for the study of lipoprotein lipase activity and lipoprotein lipase gene expression, lipoprotein lipase activity was assayed using ultraviolet spectrophotometer, lipoprotein lipase messenger RNA (LPLmRNA) levels were detected by semi-quantitative RT-PCR. blood lipid parameters in CRF rats were detected.Results: 1. Dyslipidemia occurred in the five CRF groups. SSYZ and Gemfibrozil could improve the general state, HDLc. Besides, TC, TG and LDLc concentrations were decreased (P<0.01) . There were no differentiae among SSYZ and Gemfibrozil groups. There were no dose depend effects in SSYZ groups which can increase serum ALB.2. Blood urea nitrogen contents(BUN) level, serum creatinine(SCr) level and renal lesions were occured in CC group. SSYZ had better effect on decreasing blood urea nitrogen contents(BUN) level, serum creatinine(SCr) level and on improving renal lesions than Gemfibrozil.3. The study on the LPL gene expression in heart shows: the LPL gene expression and the LPL activity of CRF rats were significantly upregulated, SSYZ and Gemfibrozil could upregulate the LPL gene expression and the LPL activity. There were no differentiae between SSYZ and Gemfibrozil groups.Conclusion: The results of the present study provide some evidences that SSYZ and Gemfibrozil can obviously regulate lipids level and improve it in renal insufficiency rats by enhance LPL activity and LPL gene expression. This effect maybe one of the mechanisms that SSYZ adjusts abnormity of lipids of chronic renal failure (CRF) rats. There were no differentia between SSYZ and Gemfibrozil groups. But SSYZ has a better certain effect on preventing nephropathy process, and raising the serum level of ALB.