A Study Of Immunoglobulin Kappa Light Chain Gene Rearrangement In The B Cell Lymphoproliferative Diseases And Its Clinical Value

Objective:To assess the clinical value of immunoglobulin Kappa light chain gene rearrangement by the method of PCR in the B cell lymphoproliferative diseases.Methods:Total 93 cases of B cell lymphoproliferative diseases and reactive lymphoid samples were detected by using the methods of touchdown PCR amplification of Ig kappa CDR3 region and heteroduplex analysis to distinguish the clonality of PCR products.Results:Ig kappa PCR demonstrated monoclonality in 34 of 72(47.2%) B cell lymphoproliferative diseases. Two of 3 cases B-CLL showed monoclonal patterns and B-ALL was 2 of 14(14.3%). Thirty of 55(54.5%)B cell non-Hodgkin's lymphoma (NHL) displayed monoclonal patterns. There was no monoclonal pattern detected in all 10 cases of reactive lymphoid samples. Successful amplification of Ig kappa was achieved in 32 of 43(74.4%)formalin-fixed and paraffin-embedded (FFPE) tissues.Conclusions: Our results showed that clonality of Ig kappa gene rearrangement could be detected by the PCR methods in B cell lymphoproliferative diseases and as a useful tumor marker, it could be used as a tool in the clinical diagnosis and differentiation diagnosis of B cell lymphoproliferative diseases. Particularly ,the clonality showed in the FFPE samples could be useful in theretrospective research.