| Objective In this experiment SD rat was used, in the process of inducing rat allergic rhinitis, on different point, the specimens with blood and nasal mucosa were obtained; by detecting the level of NO in serum and NOS in nasal mucosa, we can know the change of NO in blood and NOS in nasal mucosa with the beginning, developing and recovering of allergic rhinitis, try to find the principle of NO in allergic rhinitis. Methods Fifty SD rat were divided into five groups by random: normal group, experiment group ⑴, experiment group ⑵, experiment group ⑶, and therapeutic group were given ovalbumin (0.3mg) and Al(OH)3 (30mg), therapeutic group were given steroid seven days after successful sensitization till the symptoms of allergic rhinitis had been gone away completely. At different phase of the process in inducing allergic rhinitis we could collect veinous blood and nasal mucosa specimens; In the process, the specimens of experiment group⑴were obtained at the fourth day; the specimens of experiment group⑵were obtained at the third day after using ovalbumin in nasal cavity for irritating the nasal mucosa; the specimens of experiment group⑶were obtained when full symptoms appeared; the specimens of therapeutic group were obtained when symptoms were suppressed by using steroid. The method of inducing allergic rhinitis: It can be divided into two segments: the first segment in using ovalbumin in belley cavity by injection, once every other day, seven times at all; the second segment is using allergen in nasal cavity from the fifteenth day, once a day, seven times too. The signal of successful sensitization is these symptoms as follow: sneezing, rhinorrhea, pruritus, et. The methods of specimen obtained: ①Venous blood obtained, anesthetic was used, then cut the breast cavity open, from the right ventricle, we could obtain 1.5 ml venous blood, the blood was centrifuged by 3000-4000 revolutions per min, we got serum 0.5ml and maintained it in refrigerator(-30℃).②Nasal mucosa obtained: rats were painlessly sacrified , cut nasal cavity open, nasal mucosa could be obtained, balanced, added nine times normal saline, milled, centrifuged by 1000 revolutions per min, took the upper liquid and maintained in refrigerator(-30℃). The methods of detection: detection of NO in serum and NOS in nasal mucosa, they must be performed by using NO box, NOS box and other apparatus, strictly according to the instruction. We could get the raw materials of NO in serum and NOS in nasal mucosa. Processed by statistics: all the materials were made into uniform formula as x ±s, adopted analysis of variance and q-test between two materials, it will be significant differentwhen p<0.05 and very significant when p<0.01. Results 1. The changes of NO level in serum, experiment group⑴and⑶are very significant higher than normal group, but experiment group⑴is no different with experiment group ⑶; normal group, experiment group⑵and therapeutic group are all lower than experiment group⑴and⑶, the different are very significant, but there is no different among them. 2. The changes of NOS in nasal mucosa: experiment group⑴and ⑶, therapeutic group are all higher than normal group and experiment group⑵, experiment ⑶is the highest, the different are very significant, experiment group ⑴is no different with therapeutic group, experiment group⑵is almost the same as normal, no different. Conclusion NO is an important substance in physiology and pathology. Under normal condition, NO can maintain cillary motion, stimulate gland to secrete, the high level NO in nasal sinuses can keep the sinuses sterile; but in a allergic rhinitis patient, the level of NO is over normal, it can do harm to body. Finding a method to supervise the level of NO can let us easily control it. This experiment detected changes of NO in serum and NOS in nasal mucosa at different phase of allergic rhinitis, found that levels of NO in serum and NOS in nasal mucosa all fluctuated, and after using steroid the level became lower from this experiment we can see that the change of NO in serum is very... |
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