Study Of Differential Proteomics On Liver Cancer Cell Line And Normal Liver Cell Line

Objective To detect different protein expression in malignant and normal liver cell lines in vitro using the SELDI ProteinChip platform .which could be used as a basis to study the pathogenesis of liver cancer. Methods Two cell lines , human normal liver cell line L02 and hepatoma cell line HepG2 were cultured routinely and harvested in good conditions using cell lysis. After quantification ,the supernatant of the lysate was tested by IMAC3(Immobilized mental affinity capture) and WCX2(weak cation exchange) chip on SELDI-TOF-MS proteinchip reader respectively. Results The protein expression was different between malignant and normal liver cell lines . Total 20 different expressed proteins were found, among which ,7 were captured by IMAC3 chip and 14 by WCX2 chip. Peaks of 7979, 8492, 13428, and 15940 Da were upragulated and 8103, 10160, 11304 Da downregulated in HepG2 cells by IMAC3 chip ; 7517, 7945, 7979Da were upragulated and 5061, 5551, 5818, 8428, 10100, 10312, 11081, 11621, 11662, 11830, 12772 Da downregulated in HepG2 cells by WCX2 chip respectively. Interestingly, the 7979Da peak was captured by both chips . In addition,the 11081 Da peak was corresponded precisely to the molecular mass of calcium binding protein S100A10, which may participate in the formation of liver cancer in company with p36. Conclusion Detecting different protein expression in malignant and normal liver cell lines using the SELDI ProteinChip platform was simple ,sensitive and repeatable; the results we got could be served as a basis to study the pathogenesis and help the discovery of new therapy targets of liver cancer.