Genetic Association Of ApoE Gene, LRP Gene And BCHE-K Variant With Alzheimer's Disease In Chinese

Objective: To research the relations among apolipoprotein E(apoE) gene polymorphism ,low-density lipoprotein receptor-related protein (LRP) gene polymorphism, and Butyrylcholinesterase K (BCHE-K)variant with Alzheimer's disease (AD) in Chinese.Methods: The gene polymorphisms of LRP and apoE were genotyped in 67 AD cases and 77 controls with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods, and Butyrylcholinesterase K variant by allele-specific single step polymerase chain reaction amplifyyi- cation(AS-PCR) technique.Result: There was significant association of apoEε4 with AD (P < 0.05),AD group had higher frequencies of C/C homozygote genotype(P < 0.05) and of C allele (P < 0.05),The occurrence of the apoEe4 influence the distribution of the LRP(P < 0.05), the BCHE-K variant does not confer a risk for AD(P > 0.05). Conclusion: A positive correlation was found between apolipoprotein E gene polymorphism ,LRP gene polymorphism and AD in Chinese AD cases. No association between BCHE-K variant and AD,and BCHE-K variant does not confer a risk for AD.