The Associativity Between The Change Of Plasma Level Of Homocystein With CRP And IL-8 In Patients With Acute Coronary Syndrome

Objective: The acute coronary syndrome (ACS) is a kind of acute case of coronary heart disease (CHD) . It includes acute myocardial infarction (AMI ) and unstable angina. Plaque rupture and subsequent thrombus formation is responsible for the onset of most ACS.ACS had been a main fatal disease that influenced mankind health. To study its pathology, pathogenesis and control the risk factors is an important task nowadays for medical study. inflammation appears to play a significant role in the initiation and development of atherosclerosis. Recent investigations have shown an association between inflammatory markers such as C-reactive protein (CRP),interleukin-8(IL-8) and ACS. Homocysteine(Hcy) is a new risk factor of ACS, which differ from other conventional risk factors such as smoking, sex, age, blood pressure, diabetes, lipid disorders. However, the mechanism of Hcy inducing ACS is not demonstrated clearly. The aim of this study is to alter plasma level of homocystein by folic acid and VitaminB₁₂ and investigate the relationship between Hcy and inflammation markers CRP and IL-8, explore the possible pathogenesis mechanisms of homocystein to cause ACS and to provide clinical evidence for treatment . Methods: The study group comprised 96 consecutive patients with ACS in cardiac center of the People,s Hospital of HeBei Province, including 61 patients with unstable angina pectoris, 35 patients with acute myocardial infarction. Exclusion criteria: left ventricular ejection fraction<30%, valvular heart disease, recent operation and injury, renal or liver dysfunction, acute or chronic inflammation, acute leukemia, acute cerebrovasular disease, bronchial asthma, von Willebrand disease, cancer, oral vitamins, lack of estrogen, organ transplantation, self immunity disease. Age, sex, hypertension, diabetes, TG, TC, HDL-C, LDL-C, VLDL-C, smoking and CHD family history in every subjects were recorded in detail. Blood sampling: Peripheral blood samples were taken on the second day after the patients came into hospital .then take the blood samples again after 4 weeks,8 weeks and 12weeks respectively. Coded samples were stored at -80℃and analyzed in a single batch for the study, thus, patient management was independent of these results. Levels of Hcy, IL-8 and CRP were measured with ELISA. Statistics analysis: SPSS11.0 software pack was used to make statistical-analysis. Initially the homogeneity of variance between all the groups was analyzed. All the numerical data was shown as mean±standard deviation . students t test and analysis of variance were used to establish significance. Linear Correlation analysis was used to measure the coefficient ofcorrelation about the correlation data. We took p<0.05 as statistic signifycance level. Results: 1 There was no statistical difference between two studied group about clinical features . There was no statistical difference between two studied group with normal plasma Hcy concentration about clinical features . 2 The comparisons of Hcy,CRP and IL-8 levels in two groups. . 2.1 The levels of Hcy CRP and IL-8 were no significantly difference between treatment group and control group at baseline. 2.2 The Hcy concentrations of post-treatment were lower than those of pre-treatment in treatment group(15.28±4.71,13.17±2.79μmol/l,11.25±2.01 μmol/l), The Hcy concentrations of post-treatment were no significant lower than those of pre-treatment in control group(17.93±9.52μmol/l,17.63±8.48μmol/l, 17.68±8.5μmol/l). 2.3 The CRP and IL-8 concentrations of post-treatment were lower than those of pre-treatment in both treatment group and control group ,the lowering CRP concentration in treatment group were higher than those in control group. the lowering IL-8 concentration in treatment group were significantly higher than those in control group 3 The comparisons of Hcy,CRP and IL-8 levels in two groups with normal plasma Hcy concentration.3.1 The levels of Hcy CRP and IL-8 were no significantly difference between treatment group and control group with normal plasma Hcy concentration. 3...