Association Of CGRP, ET-1 And Plasminogen Activator Inhibitor1 Genetic Polymorphism In Chronic Pulmonary Heart Disease

Objective The aim of our study is to investigate the reason and relative gene of CPHD on molecular level by detecting the polymorphism of PAI-1 gene; and analyse the role of ET-1 and CGRP in the development of chronic pulmonary heart disease.Methods The PAI-1 genotype in 40 normal subjects and 60 pateints of CPHD is detected by the polymerase chain reaction (PCR); The level of CGRP and ET-1 in plasm of the patients and normal group is assayed by radioimmunity method.Results ①In the control group, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene is 12.5%, 72.5% and 15.0%; In the study group, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene is 15.0%, 56.7% and 28.3%; These percentages in control group do not significantly differ from the study group, 4G allele frequency and 4G/4G genotype /non 4G/4G genotype in control group do not significantly differ from the study group.②In the female control group, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene is 6.2%, 68.8% and 25.0%; allele frequency of 4G and 5G is 40.6% and 59.4% ; In the male subgroup, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene is 16.7%, 75.0% and 8.3%; allele frequency of 4G and 5G is 54.2% and 45.8%. These percentages and 4G/4G genotype /non 4G/4G genotype in female subgroup do not significantly differ from the male subgroup, 4G allele frequency in female subgroup significantly differ from the male subgroup.③In the female subgroup of CPHD, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene is 25.9%, 55.6% and 18.5%; In the male subgroup, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene is 6.1%, 57.6% and 36.3%;These percentages and 4G/4G genotype /non 4G/4G genotype in female subgroup do not significantly differ from the male subgroup, 4G allele frequency in female subgroup significantly differ from the male subgroup. ?The data of mean Hct is 0.521±0.155 and 0.519±0.097 in the CPHD group and 4G/4G genotype subgroup, respectively. In the control group, the mean Hct is 0.483±0.056. There is not significantly different in control group comparing...