| Objactive Plasminogen activator inhibitor-1 (PAI-1), a kind of glycoprotein, is a member of the SERPIN family. The important step in the progress of fibrinolysis is that tissue plasminogen activator(PA) transforms plasminogen into plasmin, the latter makes fibrin degradation. PAI-1 main function is as the fast-acting inhibitor of t-PA. This action prevents the development of fibrinolysis and makes thromsis. Angiotensin converting enzyme (ACE), membrance binding protein, is a member of peptidyl-dipeptide hydrolase. ACE transforms angiotensin I into angiotensin II. Ang II produces construction of vascular and trachea by acting on smooth muscle. The clinical studies show that most patients of chronic pulmonary heart disease(CPHD) have the syndrom of high risk blood clot and an-giotonia. We investgates the reason and relative gene of CPHD on molecular level by detecting the polymorphism of ACE gene and PAI-1 gene.Methods The ACE and PAI-1 genotype in 40 normal subjects and 60 pateints of CPHD is detected by the polymerase chain reaction (PCR).Results (1)In the control group t the distribution of the DD, II and ID genotypes of the ACE gene is 10. 0%,47. 5% and 42. 5%;In the study group, the distribution of the DD,II and ID genotypes of the ACE gene is 20. 0%,36. 7% and 43. 3%; These percentages in control group do not significantly differ from the study group, I/Dallele frequency and DD genotype /non- DD genotype in control group do not significantly differ from the study group. (2)In the control group, the distribution of the 4G/4G,4G/5G and 5G/5G genotypes of the PAI-1 gene is 12. 5% ,72. 5% and 15. 0%;In the study group, the distribution of the 4G/4G,4G/5G and 5G/5G genotypes of the PAI-1 gene is 15. 0%,56. 7% and 28. 3 %; These percentages in control group do not significantly differ from the study group, 4G allele frequency and 4G/4G genotype /non- 4G/4G genotype in control group do not significantly differ from the study group. (3)In the female subgroup of CPHD, the distribution of the DD,II and ID genotypes of the ACE gene is 25. 9% ,48. 2% and 25.9%; In the male subgroup, the distribution of the DD,II and ID genotypes of the ACE gene is 15.1%,27. 3% and 57. 6%;These percentages in female subgroup significantly differ from the male subgroup, I/D allele frequency and DD genotype /non- DD genotype in femalesubgroup do not significantly differ form the male subgroup. (4)In the female subgroup of CPHD, the distribution of the 4G/4G.4G/5G and 5G/5G genotypes of the PAI-1 gene is25. 9% ,55. 6% and 18. 5%; In the male subgroup, the distribution of the 4G/4G.4G/5G and 5G/5G genotypes of the PAI-1 gene is 6. 1%, 57. 6% and 36. 3%;These percentages and 4G/4G genotype /non- 4G/4G genotype in female subgroup do not significantly differ from the male subgroup, 4G allele frequency in female subgroup significantly differ from the male subgroup. (5)In 19 subjects of patients of CPHD accompanying with pulmonary embolism, the distribution of the DD.II and ID genotypes of the ACE gene is 31. 6%,26. 3% and 42. 1%. D/I allele frequency is 52. 6% and 47. 4%. The percentages of ACE genotypes is not significantly different in control group and subgroup, The percentages of DD genotype and non-DD genotype, the I/D alleles frequency are significant association between the two groups. (6)In 19 subjects of patients of CPHD accompanying with pulmonary embolism, the distribution of the 4G/4G,5G/5G and 4G/5G genotypes of the PAI-1 gene is 10. 5 % ,21. 1 % and 68. 4 %. 4G/5G allele frequency is 44. 7 % and 55. 3 %. The percentages of PAI-1 genotypes, the percentages of 4G/4G genotype and non-4G/4G genotype, the 4G/5G alleles frequency are not significantly different. (7)The data of mean Hct is 0. 521±0. 155, 0. 528±0. 112 and 0. 519 ± 0. 097 in the CPHD group, DD genotype subgroup and 4G/4G genotype subgroup. In the control group, the mean Hct is 0. 483 ± 0. 056. There is not significantly different in control group comparing with CPHD group, DD genotype subgroup and 4G/4G genotype subgroup.Conclusion (1)...
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