| Objective : In this study,we used propofol on the model of lung injuryfollowing hind limbs ischemia/ reperfusion in rats to observe the changes ofMDA content,SOD activity,and the expression of inducible nitric oxidesyntheses(iNOS) , intercellular adhesion molecule typel (ICAM-1) in lung tissueand combined with the changes of pathology in lung tissue to discuss the organprotective effects of propofol and its mechanism. we hope this study will offerthe experiment support for the application of propofol on organ protection inclinical practice . Methods : Twenty-four healthy male SD rats weighing 250-300g wererandomly assigned into three groups and anesthetized by 25% urethane 1.2g· kg-1, i p.:(1)sham group (n=8) , the rats received the operation without othertreatment;(2) hind limbs ischemia / reperfusion group ( I/R group , n=8) , hindlimbs 3- hour ischemia followed by 4-hour reperfusion ; (3) propofol treatedgroup (I/R+P group , n=8) , the rats received a bolus of 0.5 % propofol5mg·kg-1 , iv and then continuously infused 10mg . kg -1 . h1-,10 minutes beforereperfusion . The I/R group were administered with an equivalent dose ofnormal saline ( NS ) alone instead of propofol , that is 1ml·kg-1 bolus followedby 2ml·kg-1·h-1 continuously . At the end of the experiment , all rats werekilled by carotid bloodletting , the lung tissue was sampled to determined theMDA content , SOD activity , the water content of lung , the expression ofiNOS , ICAM-1 and have microscopic examination.Result:1.The variety of MDA in lung tissue follow : The MDA level of lung in groupI/R (1.35±0.468nmol/mg prot) were notably higher than that in group sham(0.83±0.16 nmol / mg prot ) (P<0.01) . Compared with the group I/R , the MDAof lung MDA (0.856±0.275 nmol/mg prot ) (P<0.01) decreased obviously ingroup I/R+P (P<0.05). After compared I/R+P group with sham group , we foundthat the content of MDA in the lung tissues had no difference by statisticsanalysis (P > 0.05) .2.The alteration of SOD activity in every tissueThe SOD activity of lung was 12.681±1.732 NU/mg prot in sham group . Itwas 7.512±2.153NU/mg prot in group I/R ( remarkably lower than group sham ,P<0.01) . It was higher in group I/R+P (9.926±2.568NU/mg prot) than that ofin group I/R(P<0.05) , but lower than in that of group sham (P<0.05).3. The variety of water content in lung (%) The pneuma water content of group sham , group I/R and group I/R+P were(62.8±8.35)% , (76.2±6.23)% ,and (68.9±7.12) % respectively . The pneumawater content of group I / R was largely increased compared with that of groupsham (P<0.01) .This index was reduced in group I/R+P contrasting with groupI/R (P <0.05) . And it had no statistic discrepancy in group I/R+P and groupsham (P >0.05)4. histological and pathological changes in lung tissue Light microscopy findings in I/R group included alveolar edema,localpulmonary ateletasis hemorrhage,thickening of interstitialand large amount ofpolymorphomiclear(PMN) infiltration . In contrast , these changes were lessprominent in the rats receiving propofol .5 the expression of iNOS and ICAM-l Compared with the sham group the expression of iNOS and ICAM-1 in I/Rgroup increased obviously in lung tissue and propofol attenuated this increase inI/R+P group . Conclusion:The experiments presented here provide evidence thatpropofol have protective effect on lung injury following ischemia / reperfusionof hind limbs in rat. Elucidation of the details by which this pathway is highlylikely that the protective mechanism of propofol attenuated generation ofreactive oxygen species and relieve secondary inflammatory. Post-ischemicpharmacological interventions with propofol that reduce lung injury . Thisfurther emphasizes the therapeutic significant of propofol in preventionischemia – reperfusion of lung injury . |
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