The Study On Changes Of IGF-Ⅱ And IGFBP-3 In Cerebrospinal Fluid Of Children With Central Nervous System Infection

Objective: The insulin-like growth factor system include 2insulin-like growth factors (IGF): IGF-â… IGF-â…¡and 6 insulin-likegrowth factor binding proteins (IGFBP): IGFBP-1_IGFBP-6.Insulin-Like growth factors who share structural hormology with proinsulinstimulate cellular proliferation and differentiation. Circulating IGFS,primarily liver derived, act in an endocrine fashion, whereas mostother tissues locally synthesized IGFS act in a paracrine fashion orautocrine fashion at local receptors. The locally synthesized IGFS alsocan act in an endocrine fashion at other tissues. IGFS mostly bound toIGFBPS, which may translate IGFS to their target tissues and modulatethe biological actions of IGFS. The major IGF in cerebrospinal fluid(CSF) is IGF-â…¡and the major circulation IGFBP in serums isIGFBP-3, whereas IGFBP-3 is only a minor component in thecerebrospinal fluid of health subjects but may be increased inpathological states. Insulin-like growth factor system plays animportant physiological role in brain and are involved in thepathological process of many central nervous system diseases. Wemeasured changes of insulin-like growth factor-â…¡(IGF-â…¡) andinsulin-like growth factor binding protein-3 (IGFBP-3) incerebrospinal fluid in children with bacterial meningitis tubercularmeningitis or viral meningitis and study their roles in central nervoussystem infection.Methods: The levels of IGF-â…¡and IGFBP-3 were measuredby Enzyme Linked Immuno Sorbent Assay (ELISA). CSF sampleswere obtained from 45 children with central nervous system infection:15 cases with bacterial meningitis, 15 cases with tubercular meningitis,15 cases with viral meningitis .CSF control samples were obtainedfrom 15children without neurologic diseases.Results: The levels of IGF-â…¡were markedly different in the CSFof patient with bacterial meningitis tubercular meningitis and viralmeningitis. The levels of IGF-â…¡were markedly higher in the CSF ofpatient with bacterial meningitis than patient with tubercularmeningitis (t=8.05, p<0.001) viral meningitis (t=14.14, p<0.001) andcontrol group (t=15.36, p<0.001). IGF-â…¡levels of patient withtubercular meningitis were markedly higher than the levels of viralmeningitis group (t=5.11, p<0.001) and control group (t=6.21,p<0.001). The levels of IGF-â…¡had not obvious distinction betweenviral meningitis group and control group (t=1.32, p>0.01). The levelsof IGFBP-3 were markedly different in the CSF of patient withbacterial meningitis tubercular meningitis and viral meningitis. Thelevels of IGFBP-3were markedly lower in the CSF of patient withbacterial meningitis than patient with tubercular meningitis (t=6.73,p<0.001) and were markedly higher than viral meningitis (t=7.11,p<0.001) and control group (t=8.10, p<0.001). IGFBP-3 levels ofpatient with tubercular meningitis were markedly higher than the...