Experimental Study Of Inflammation Mechanism In Carotid Restenosis

Objective: To investigate the mRNA expression of MCP-1 and IL-8 in karyocytes and dynamic changes of Mac-1 and LFA-1 expressed on monocytes in peripheral vein blood in process of canine carotid restenosis model establishment with angioplasty.Methods: Ten majority canis familiaris were selected to establish carotid restenosis animal models with angioplasty under digital subtract angiography. Peripheral vein blood was collected before the procedure and at the 2~nd hour. 4~th hour. 6~th hour. 12~th hour. 24~th hour. 48~th hour. 72~nd hour. 1~st week after angioplasty. The dogs were sacrificed at the eighth week and the target carotids were collected to perform pathology. The mRNA expression of MCP-1 and IL-8 in karyocytes was qualitatively detected with RT-PCR; the expression levels of Mac-1 and LFA-1 on monocytes were measured with flow cytometry. The data were statistically analyzed with SPSS 10.0. The value of P less than 0.05 was considered significant.Results: 1.The procedures of all canine carotid restenosis modelsestablishment were successful. Angiography was performed at the eighthweek after angioplasty and the stenosis rate was 65.00±15.47%. Thepathology of target carotids supported restenosis. 2. Neither MCP-1 nor IL-8 mRNA expression in karyocytes was detected before angioplasty. The mRNA expression of MCP-1 was first detected at the 24th hour and the mRNA expression of IL-8 began to be detected at the 48th hour. At the 1st week, both mRNA expression of MCP-1 and IL-1 could still be detected. 3. The expression levels of Mac-1 on monocytes at different time point were significantly different under the analysis of variance (F=9.506, P<0.01). Compared with the expression level of Mac-1 on monocytes before angioplasty, the expression levels of Mac-1 at the 12th hour, 24th hour, 48th hour and 72nd hour after angioplasty were significantly increased in peripheral vein. The expression levels of Mac-1 showed no significant difference between the 12th hour and the 24th hour (P>0.05); compared with the expression level of Mac-1 at he 24th hour, the expression level at the 48th hour was significantly increased; however there was no significant difference between the expression level at the 48th hour and at the 72nd hour; and there was no significant difference between preoperation and the 1st week after the procedure. The expression levels of LFA-1 on monocytes at different time point showed no significant difference under the analysis of variance (F=1.553,P>0.05).Conclusions: The carotid restenosis model of canine established with angioplasty is a stable animal model. In the process of restenosis formation after angioplasty, MCP-1 IL-8 and Mac-1 may all play an important role byparticipating the acute system inflammation, and also MCP-1 and IL-8 by participating the chronic system inflammation.