To Study The Etiology Of Children's Viral Encephalitis In Guangzhou

Objective:1 To study the pathogenic and epidemic characteristics of children's viral encephalitis in Guangzhou.2 To investigate viral encephalitis caused by the coltivirus in Guangzhou Methods1. Viral DNA/RNA in CSF from 198 patients with VE and 100 patients with other CNS diseases were detected by FQ-PCR and IgM antibodies were determined by ELISA.2. Coltivirus antibody IgG of paired-serum from 65 patients with viral encephalitis and 50 patients with other CNS disease was tested by ELISA and antibody of Japanese encephalitis were determined by RPHIResult1. The pathogen were tested positive in 66 of 198 patients with VE , in whichEV was 25cases,HSV- I 19cases, HSV- II 1 lcase, CMV 3 cases, IFV 1cases, ADV 5cases, JEV 6cases, RSV 2cases, EBV 3 cases. No MV was detected.100 patients with other CNS diseases were tested negative.2. Viral encephalitis can occurred all the year and prevailed from May to October. The encephalitis caused by EV and JEV that younger children are subject to were occurred in summer and autumn ; the encephalitis caused by HSV had not obviously seasonal and affected the older children.3. The coltivirus antibody IgG was tested positive in 7 of 65 paired-serum in the group of VE, the positive rate was 10.7%; while 2 case of 50 paired-serum in the group of other CNS diseases were tested positive and positive rate was 4%.Conclusion1. The major pathogens caused viral encephalitis were the EV and HSV in Guangzhou. The epidemic seasons of encephalitis caused by EV which affected the younger children was in summer and autumn; while encephalitis caused by HSV was not a seasonal disease and affected the older children.2. Coltivirus probably can cause encephalitis in Guangzhou.