Study On Correlation Of P33～(ING1), P53 And P21～(WAF1) In Cervical Cancer

Cervical cancer is a kind of Women malignant neoplasm with a high incidence, its occurrence and development is a complicated multi-steps biological behavior involving multiple factors, including many kinds of anti-oncogene inactivation and oncogene activation. There are few anti-oncogene to be separated and appraised because of its recessive characteristic and difficult separating and appraising method. But the anti-oncogeuces are one of the most important factor to resist carcinogenesis. The most stress one is p53, A large number of investigations showed that there were p53 mutations in about 50% human cancers. Its expression and regulation is correlated with p21^WAF1 and p33^ING1. ING1 is a novel anti-oncogene, it can help p53 gene to resist carcinomas. ING1 was copied by Garkavtsev in 1996 with genetic suppressor element method(GSE). It can inhibit the growth of cells, and was named " inhibitor of growth (ING)" . Researchers in our country and abroad have been detected abnormal expressions of PBS^ONG1 in stomach cancer, liver cancer, large intestine cancer, bladder cancer, melanoma and glioma etc. But there is no report about P33^ING1 in cervical cancer.Objective: To disscuss expression of P33^ING1, P53 and P21^WAF1 in cervical cancer and its relationship, with the expression of P53 and P21^WAF1.Material and Method: The study population consisted f cervical specimens from the department of Pathology, the Third Affiliated Hospital of Zhengzhou University from January 2000 to June 2004. 148 cases of cervical specimens include:30 normal cervical tiusses; 18 atipical hyperplasia; 21 carcinoma-in-situ and 69 cervical invasive cancer(all squamous carcinoma). None of cervical cancer patient accepted chemotherapy and radiotherapy before operation. In this study, we use immunohistochemical method to detect the expression of P33ING1 > P53 and P21WAF1 in 148 cases of cervical specimens in order to analysis the changes of P33 ' P53 and P2j\vafi jn ^ cerv}cai cancer occurrence and improvement, and try to get a new clinical marker to evaluate the risk of cervical carcinogenesis and prognosis of the carcinoma.Result: 1. Positive rates of PSS1*01 are 100%> 72.0%, 57.14% and 59.42% in normal cervical tiusses> atipical hyperplasia^ carcinoma-in-situ and cervical cancer. There are significant difference between any two groups, but the last two.2. Positive rates of P53 are 0, 0, 28.57% and 59.42% in normal cervical tissues> atipical hyperplasia ^ carcinoma-in-situ and cervical cancer. There are significant difference between any two groups, but the first two.3. Positive rates of P21WAF1 are 80.00%, 61.11%, 47.62% and 28.99% in normal cervical tissues^ atipical hyperplasia^ carcinoma-in-situ and cervical cancer. There significant difference between any two groups.4. In the cervical cancer, the expression of P33ING1 is negative correlated with P53.5. In the cervical cancer, the expression of P33ING1 is positive correlated withp21WAfl_Conclusion : 1. ING1 is a tumor suppressor gene, its expression in cervical cancer is low and plays an important role in occurrence and improvement of cervical cancer.2. In the cervical cancer, the expression of P33ING1 is negative correlated with P53, p33ING1 is negative factor to inhibit growth of different cell lines. The properties of p33ING1 suggest its involvement in negative regulation of cell prliferation and in the control of cellular aging, anchorage dependence and apoptosis, it may be a companion gene of p53.3. In the cervical cancer, the expression of P33ING1 is positive correlated with P2jWafi j^Q activation of transcription from the p21WAF1 promoter, a key mechanism of p53-mediated growth control, depends on the expression of pSS?01. The pS was shown to inhibit cell growth through the activation of p21WAF1 with p53.