| Objective: To study the effect on endocrine hormone and immunology function in rats dams and offsprings after pregnant rats exposure to cypermethrin and methylparathion.Method: pregnant Wistar rats were divided randomly into 4 groups. Three exposure groups had been force-fed once per day from first day to 15th day during gestation period using 1/300 LD50, 1/95 LD50, 1/30 LD50 cypermethrion mixed equivalent doses of methylparathion, respectively. Pesticides were dissolved in edible oil. One control group was force-fed solvent only. The half pregnant rats were killed when they had been pregnant for 19 days and the viscera relative weight were measured. The serum genital hormone Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), Estradiol(E2)and Testosterone(T); the serum thyroid hormone Triiodothyronine (T3), Tetraiodothyronine (T4) and Thyroid stimulating hormone (TSH); IgG and IgA level were measured using Radioimmunoassay (RIA). The other pregnant rats gave birth to child naturally. Their offsprings' body weight, body length, tail length and the time of auricle separation, teeth burgeon, eye opening, ear hole opening, hair appearance were measured. Then 64 offsprings (8 each group, half female and half male) were killed when they were 70-day-old and the same indexs as pregant rats' were measured.Results: The pregnant rats' relative weight of liver were decreasing along with pesticide dose going up(r_s= -0. 2819, P=0. 0071); the pregnant rats' relative weight of thyroid gland were increasing along with pesticide dose going up(r_s=0.2715, P=0. 0096);the mean serum T3 level of pregnant rats in dose of 1/95 LD50 were higher than that of control group (P<0.05), and there was a dose-effect relationship (r_s= 0.3410, P=0.0072); the mean serum T4 level of pregnant rats were increasing along with pesticide dose going up(r_s= 0. 2875, P=0. 0247). The offsprings' tail length 1/30LD50 were longer than that of control group (PO.05), and there was not a dose-effect relationship (rs= 0.0696, P=0.1809). The mean serum T level of male offsprings in dose of 1/300 LD50 were higher than that of control group (PO.05), and there was not a dose-effect relationship (r,=-0.1120, P=0. 3900).Conclusion: Our results showed that Wistar pregnant rats exposure to the combined pesticides of cypermethrin and methylparathion for 15 days had such effects: In dose of 1/95 LD50, the serum T3 increasing in pregnant rats; In dose of 1/30 LD50, the offsprings' tail length increasing;In dose of 1/300 LD50, the serum T increasing in male offsprings. Our results showed that combined pesticides had affected the pregnant rats' and the offsprings' endocrine hormone. But we did not found that combined pesticides affected the offsprings' immunological function.
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