Expression Of Survivin,CD44v6,MMP-2,TIMP-2 And P27 In Cervical Carcinoma And Their Relationship With The Invasion And Metastasis Of The Cancerous Tissues

Objective To investigate the expression of Survivin CD44v6 . MMP-2 TIMP-2 and P27 in cervical carcinoma and their relationship with the invasion and metastasis of the cancerous tissues.Methods The expression of survivin CD44v6 MMP-2 TMP-2 and P27 were determined by immunohistochemical S-P method and colour pathological image computer analysis system, 10 cases of normal cervical epithelia,10 cases of cervical carcinoma in situ,40 cases of invasive squamous cell cervical carcinoma and 11 cases of invasive cervical adenocarcinoma were included in this retrospective study.The relationship between the expression and clinical pathology of cervical carcinoma was analyzed statistically.Results It was found that the positive level of survivin CD44v6 and MMP-2 expression increased in the order of normal cervical epithelium,cervical carcinoma in situ and invasive carcinoma of cervix (P <0.05),but there was no statistical difference of TIMP-2 level between them. There was significant correlation between the expression of survivin CD44v6 MMP-2 TIMP-2 and P27 and pelvic lymphonode metastasis,intravascular involvement,stroma involvememt and histropathological types and ages of patients(P<0.05),but there was no correlation between them and FIGO staging and histropathological grading (P>0.05).The positive expression of survivin CD44v6 MMP-2 and TIMP-2 in patients unter 35 year old or with pelvic lymph node metastasis,intravascular involvement and stroma involvement was significantly higher than that in the cases without them,while TIMP-2 expression was opposite to that of MMP-2(P<0.05).Survivin was expressed significantly higher in squamous cell carcinoma cases than in andeocarcinoma cases, but MMP-2 and TIMP-2 was expressed higher in andeocarcinoma cases.Conclusion The positive expression of survivin CD44v6 MMP-2 TIMP-2 and P27 in invasive carcinoma of cervix might play an important role in the development, lymph node metastasis,intravascular and stroma involvement of cancer cells,and they had affinity with clinical behavior of cervical carcinoma.