Research On HLA-A,B,DR Gene Frequency With Idiopathic Nephrotic Syndrome

Objectives: We choosed 30 patients with idiopathic nephrotic syndromes(INS)of Han Population of people in Shanxi and 45 healthy people in order to access the association of HLA-A,B,DR gene polymorphism with,to look for susceptible HLA genes to INS's patients and the HLA gene frequency in INS's patients, and to investigate the etiopathogenisis and pathogenesis of INS.Materials and Methods : 30 patients were selected ,those who were with steroid-resistant nephrotic syndrome (SRNS) from department of nephrology of the first affliated hospital of Shanxi Medical University in 2005. Out of 30 patients, 22 are adults and 8 are children and renal biopsy was performed in 22 adults patients, 10 of whom showed IgA nephropathy (IgAN), 5 of whom showed mesangial proliferative glomerulonephritis (MsPGN), 4 of whom showed membrane proliferative glomerulonephritis (MPGN), 3 of whom showed membranous nephropathy (MN). whereas 45 healthy people without Nephrotic Syndrome are in the control group.The alleles of HLA-A,B,DR loci in 23 patients and in 45 healthy ones in control group were subtyped by polymorase chain reaction with sequence specific primer (PCR-SSP) and the alleles of HLA-A,B,DR loci in 7 patients were subtyped by polymorase chain reaction with sequence specific oligonucleotide probe (PCR-SSO). Results are analyzed between HLA-A,B,DR allelic frequency of SRNS and those gene frequencies of healthy control.HLA-A,B,DR allelic frequencies are analyzed in 30 SRNS patients, 22 adults patients, 8 children patients and 10 IgAN patients and in 45 healthy controls one by one.Results In SRNS group there were firstly 12 HLA-A alleles were observed by 23 specific primers and the range of allelic frequencies is from 1.67% to 31.67%. Among of them the HLA-A*02,*24,*11 allele are popular in the patients-----the frequency of them is 31.67%,16.67%,15.00% respectively , and the HLA-A*01,*29,*30,*32 are rare in the patients---the frequency of them is 1.67%. Secondly 17 HLA-B alleles were observed by 47 specific primers and the range of allelic frequencies is from 1.67% to 23.33%. Among of them the HLA-B*15,*44 allele are popular in the patients ---the frequency of them is 23.33%,20.00% respectively, and the HLA-B*07,*08,*37,*38,*53 are rare in the patients– all the frequency of them is 1.67%. Furthermore 12 HLA-DRB1 alleles were observed by 23 specific primers and the range of allelic frequencies is from 1.67% to 21.67%. Among of them the HLA-DRB1*04,07,09 allele are popular in the patients---the frequency of them is 21.67%,16.67%,16.67%...