Study On The Distribution Of Sappan Wood Ethylacetate Extract In Donor's Heart Of The Cardiac Allograft Rats

Objectives: Through studying the distribution of Sppan acetic ether extract in donor's hearts of rats and its effect on IL-1β in blood, to approach the distribution target ability of sappan in donor's hearts, and its possible immune resist mechanism.Methods: 40 mice were divided into 5 groups randomly: blank group, CsA group, sappan high, normal and low dose groups, and DAS-ELISA was adopted to detect the IL-1β activity in blood. Improved Ono model was used to replicate the heart transplantation model of rats. Successfully operated rats were parted into 2 groups: model group and sappan group. Transmigrated constituents of sappan in transplanted hearts were observed by HPLC-FPC. The change of cardiac muscle histomorphology were observed.Results: Sppan extract degraded IL-1β activity of mouse blood. Chromatogram of transplanted hearts proves that component in 5.217min is sappan original substance, and the rests are its metabolite. There are three Sppan metabolites in orthotope and transplanted hearts, and the contents are higher in the later. The extract could lessen pathological damage of transplanted cardiac muscle of rats.Conclusions:The Sppan extract could degrade IL-1β of blood, indicating that suppressing IL-1β activity may be one way of its immunosuppressive action. Its prime constituents appeared in chromatogram of transplanted hearts, but not detected in normal hearts. Sppan metabolite contents in transplanted hearts is higher. So the sappan constituents may have the target ability in transplanted heart. This extract could lessen pathological damage of transplanted cardiac muscle of rats.