| Objective To investigate the effect of atorvastatin on urinary protein excretion, a-smooth muscle actin(a-SMA),transforming growth factor beta-1(TGF-β1) and plasminogen activator inhibitor 1(PAI-1) expression in experimental mesangial proliferative glomerulonephritis(MsPGN) induced in rats, and to explore the possible mechanisms for renoprotection.Methods MsPGN was induced in Sprague-Dawley rats by intravenous injection of rabbit anti-rat thymocyte serum(ATS). Rats were randomly divided into normal control group, glomerulonephritis group, low-dose group(8mg/kg.d) and high-dose group(16mg/kg.d).After 12 days of therapy,serum total cholesterol, triglycerides, creatinine and 24 hours urine protein excretion were measured using automatic biochemistry analyzer. Renal mesangial collagen fibers were stained with Picric acid-Sirius red and collagen volume fraction(CVF) was then calculated. The a-SMA expression was assessed by immunohistory chemistry. Renal expressions of TGF-β1 mRNA and PAI-1 mRNA were detected by RT-PCR.Results (1) The 24-hour proteinuria excretion in the model group was significantly increased in contrast to the normal group(P<0.01).After therapy the 24-hour proteinuria excretions in therapy groups were reduced in a dose-dependent manner in contrast to the model group(P<0.05),low-dose group(21.17±0.79)mg/d ,high-dose group(9.77±0.54 mg/d) vs model group(30.34±0.62)mg/d. And there were no significant changes in serum total CHOL and TG.. (2) Renal histological damage in therapy groups was decreased significantly compared with the model group.Mesangial cell proliferation was suppressed and... |
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