| Objective To study the effect of selection technique on sperm concentration sperm progressive motility, abnormal sperm morphology, sperm hypo-osmotic test percentage sperm acrosin activity and they association with the fertilization rate of IVF . methods the male partners from 53 infertility couples who were selected IVF, semen sample had been collected on the day of oocyte retrieval, routine sperm parameters sperm Hypo-osmotic test percentage and sperm acrosin activity were assessed on semen specimen of a freshly liquefied and after its Selection by puresperm density centrifugation techniques. The two specimen analyses were based on WHO guideline , spermatozoa were counted using a markler chamber under phase-contrast microscopy , percentage of progressive motile spermatozoa were assessed on fields of the counting chamber, thin smears stained with modified papanicolaou staining methods were prepared for sperm morphology and examined with microscopy obtain the percentage of abnormal sperm morphology, Hypo-osmotic swelling test was carried out to obtain the percentage of spermatozoa that reacted in the HOST, sperm acrosin activity was determined spectrophotometrically according to a modified method of kennedy et al. to study the effect of selection technique on sperm concentration sperm progressive motility, abnormal sperm morphology, sperm hypo-osmotic test percentage, sperm acrosin activity and they association with fertilization of the IVF. result the sperm progressive motility, percentage of abnormal sperm morphology sperm hypo-osmotic test percentage and sperm acrosin activity was significantly changed after selection technique ( p<0.000 ). sperm count , sperm progressive motility, percentage of abnormal sperm morphology and sperm hypo-osmotic test percentage was not significantly association with fertilization rate of the IVF; sperm acrosin activity was...
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