Affects Of Anesthetic Schemes On The Expression Of Interlukin-1β And Interlukin-6

Objective The study was consisted of two parts : Partâ… To compare sthe changes of interleukin-1β(IL-1β) and interleukin-6(IL-6) in epidural block and general anesthesia; Partâ…¡To evaluate the effects of different anesthetics on IL-1βand IL-6.Methods Partâ… Twenty ASAâ… -â…¡patients who underwent elective article caput femoris replacement surgery were randomly divided into epidural block group and general anesthesia group. Blood samples were taken before anesthesia(T₁), 30min after skin incision(T₂), 1hour after skin incision(T₃), at the end of surgery(T₄), and on the morning of post-operative day 1 and 3(T₅,T₆) for determination of plasma IL-1βand IL-6 concentrations; Partâ…¡PBMCs were separated from the whole blood obtained from 6 healthy persons by Ficoll method. This part included 8 groups: group 1: phosphate buffer solution was added to PBMCs; group 2: PBMCs were stimulated by lipopolysaccharide(LPS) 1μg/ml; group 3-8:different anesthetics (propofol 4μg/ml, ketamine 250μg/ml, fentanyl 5ng/ml, midazolen 500ng/ml, lidocaine 2μg/ml, bupivacaine 500ng/ml ) were added to LPS stimulated PBMCs. IL-1βand IL-6 productions were measured by using ELISA.Results (1) Plasma IL-6 concentrations increased significantly in both groups, then achieved to the apex at the end of the operation, and maintained until the morning of post-operative day 1. Plasma IL-6 concentrations were higher in epidural block group than in general anesthesia group at T2-T5, but there was no significant difference from the expression of IL-6 in both groups. IL-1βwas not tested in both groups. (2) Therapeutic concentrated propofol and ketamine inhibited the increased production of IL-6 which was produced by the LPS stimulated PBMCs, and ketamine also inhibited the expression of IL-1β. There were no significant effects to low concentrated fentanyl, midazolen, lidocaine and bupivacaine in the expression of IL-1βand IL-6.Conclusion (1) Surgical operation enhanced the production of IL-6, different anesthesia methods have few effects on the expression of IL-6.(2)Propofol and ketamine inhibited the increased production of cytokines which were produced by the LPS stimulated PBMCs,...