Association Of Polymorphism Of MTHFR Gene And Plasma Homocysteine With Stroke In Chinese Youth And Middle-aged Adults

objective :To investigate whether the genetic mutations of methylenetetrahydrofolate reductase(MTHFR)C667T and elevted plasma total homocysteine (tHcy) increase risk of stroke in Chinese youth and middle-aged adults.Methods 80 patients with stroke and 60 controls were recruited MTHTR genetic C667TPolymorphism was determined By PCR-RLFP,Plasma total homocysteines levels were measured with high performance liquid chromatography .Date of medical history,physical examination and some laboratory examination were also collectedResult the frequence of homozygous and heterozygous MTHFR mutation in stroke patients were higher than in controls (76.3% versus55.0% p=0.009)and frequency of T aleles in stroke patients was higher than in controls (55.05% versus37.5 %,P=0.023) the mean plasm tHcy and incidence(%)of hyperhomocysteinernia and were significantly higher in stroke group than contol group(26.52±18.03(75%)versus 13.95±4.05(21.7%);p<0.01) The tHcy concentration was significantly higher in persons with TT genotype than in those with CC or CT and TT>CT genetype>CC genetype Multiple stepwise logistic regression analysis showed that elevated tHcylevels wasl significantly associated with stroke after adjusted the conventionl risk factorsconclusion: Hyperhomocysteinemia is an independent risk factor of stroke in Chinese youth and middle-aged adults, Genetic mutations of MTHFR C677T is possibly important mechanism of hyperhomocysteinemia。...