An Experimental Study On The Effect Of Kangxin Liquidon Brain Mitochondrial DNA Deletional Mutation In Aged Balb/C Mice

Objective:To explore the mechanism of KangXin liquid(a prescription for tonifying Kidney and Spleen, nourishing,removing blood stasis) in retarding aging by investigating the effect of KangXin liquid on Brain mitochondrial DNA(mtDNA) deletion in aged Balb/c mice, to provide the theoretical basis for new drugs of Chinese traditional medicine's research and development.Method:1 The brain mtDNA of the young Balb/c mice(6weeks),middle age Balb/c mice(6months)and aged Balb/c mice (14 months) were isolated respectively.By using polymerase chain reaction (PCR) technique to examine the fragment deletion of Brain mtDNA.2 In our natural aging model ,the mice were divided into two groups,normal aged controlled group giving 0.9% NS ,KangXin liquid group giving KangXin liquid.After four monlhs,lhc brains of mice were deleted.3 The deletions of Brain mtDNA were detected by using PCR and electrophoresis. Analysis of deletions was proceeded in two different-group mice.Results:1 There were 304bp mtDNA deleted in brain mitochondrial of aged Balb/c mice,but same matDNA deletions were not detected in brain mitochondrial of young and middle age mice.2 Compared with aging controlled group,KangXin liquid could reduce the mitochondrial DNA deletions in brain mitochondrial of aged mice.Conclusion:1 mtDNA deletions are possible and could accumulate with age.2 KangXin liquid can obviousy ingibit the increase of level of brain mtDNA deletions to retard aging in aged mice.The conduction for prescription of tonifying the kidney and spleen,nourishing,activating blood is an ideal way for retard aging.