Anti-Anxiety Effects Of Egg Oil Of Rana Temporaria Chensinensis David

The anti-anxiety effects of egg oil of Rana temporaria chensinensis David (EORTCD), the liposoluble part extracted from Rana temporaria chensinensis David by supercritical fluid extraction of CO₂ and the mechanism of EORTCD were studied preliminarily.EORTCD at doses (2.0 ～ 4.0g · kg^-1) i.g. significantly prolonged the stay time of rats and increased the stay numbers of rats in open-field ,but the parameters were not influenced remarkably in close-field in the elevated plus-maze test(EPM),which indicated the anti-anxiety effects of EORTCD. Additionally, products of supercritical fluid extraction of CO₂ was as effective in the staircase test as in EPM. They caused a decrease in the number of rearings (NR) without changes in the number of steps ascended (NSA). Moreover, time-efficacy relation of anti-anxiety effect of EORTCD revealed the activity was the strongest at 30 min to 60 min. EORTCD notably shortened the motionless time in forced-swimming test and showed antidepressant effect. Percentage of active avoidance response and incubation period entering the dark room were reduced markedly in step-down test in rats and step-through test in mice administrated by EORTCD. Step-down latency (SDL) was reduced, number of errors (NOE) was increased in 24h and escape latency (EL) was prolonged in 48h in step-down test in mice. The results manifested the effects of EORTCD on memory of rats or mice may be significant.Writhing times was decreased in writhing test and ache threshold value was raised. Sleep latency was significantly shortened and sleep time was prolonged in the sleep test induced by ethanol. The biological activity of EORTCD was also evaluated by convulsant model induced by pentylenetetrozole and strychnine and spontaneous activity of mice. EORTCD enhanced the dosage of convulsion induced by pentylenetetrozole, and prolonged the latency of convulsion and demonstrated a relatively strong inhibition to locomotor activity. The results lengthen the analgesic and anti-convulsion effect. In climbing wire netting test, the falling time...