The Active Component Abstrcation Analysis Of Rhizoctionic Leguminicola And It's Secondary Metabolite

Aim:The purpose is to analyze the component of Rhizoctonia leguminicola mycelium and its secondary metabolite,find its medicinal properties and develop new uses of Rhizoctonia leguminicola.Methods:1. Inoculating Rhizoctonia leguminicola on PDA in a stated time,making it holding hearty vitality. 2. Certain strains were cultured on Czapek's medium.Rhizoctonia leguminicola mycelium and its culture solution were obtained. 3. Treating mycelium and secondary metabolite separately with different methods.①The treatment of mycelium: the naturally dried mycelium was dipped in 0.5% hydrochloric acid for 24 hours and then treated with Ultrasonic cleanser in 40KHz at 70℃for 30 minutes.It was repeated for 3 times.These extracted solutions were combined and then condensed decompressedly at 60℃.Then adjust the solution to pH2 with 2 mol/L hydrochloric acid,join fresh ammonium reinecke,ammonium tetrathiocyana todiammine chromate saturated solution, gather deposite and dissolve it in the acetone,filtrate infusibility matter,join silver sulphate saturated solution to filtrate.After filtration,join mensurable barium chloride solution to it, adjust the filtrated solution to pH10 with 2 mol/L ammonia.Then the solution was aired and brown ropy matters with special smell were obtained.②the treatment of culture solution:the first method was as follows.About 1000 mL pretreated culture solution were condensed decompressedly at 60℃.The organic phase and acid solution were obtained by means of extracting the condensed solution with butanol for 10 times after adjusting it to pH2.The acid solution was adjusted to pH10 and then extracted with butanol for 10 times. It contained water and organic phase.The new water phase was obtained after extracting organic phase with 0.2 mol/L dilute sulphuric acid.It was condensed after treated with strong ammonia and then sublimated decompressedly.There appeared white powder.The second method was as follows.Taking 1000 mL pretreated culture solution of Rhizoctonia leguminicola,adjust to pH10,shaken(3*) with H2O:CH2CL2(v/v),the water extract was adjusted to pH7 and absorbed on a strong cationexchange resin(H+), eluted with 1 mol/L NH4OH,and the filtrate was dried.The residue was adjusted to pH7 and lyophilized to obtain a light yellow powder.There...