Study On The Expression Of P-gp, GST-Ï€ And TS Proteins In Cervical Cancer And Their Relationship With ATP Tumor Chemosensitivity Assay

Objecitive(1) To investigate the expression of drug resistance-associated proteins P-gp, GST-Ï€ and TS in primary cervical cancer and their clinical significance.(2) To explore the feasibility of the ATP-tumor chemosensitivity assay ( ATP-TCA) applied in human cervical cancer chemosensitivity testing.(3) To analyse the relationship between the three drug resistance-associated proteins and ATP-TCA.MethodsConsecutive sections from 51 cases of cervical cancer and 9 normal cervical tissues were assessed immunohistochemically for expression of P-gp, GST-Ï€ and TS proteins. ATP-TCA was used to detect the sensitivity of 35 specimens of fresh cervical cancer to six cytotoxic drugs as follows: paclitaxel (TAX), cisplatin (DDP), bleomycin (BLM), gemcitabine (GEM), 5-fluoruracil (5-Fu), irinotecan (CPT-11).Results(1) P-gp and TS proteins were not detectable and high expression of GST-Ï€ protein was observed in 4 (44.44%) of 9 normal cervical tissues. Positive expression of P-gp protein was observed in 23 (45.10%), high expression of GST-Ï€ protein in 32 (62.75%), and TS protein in 22 (43.14%) of the 51 tumors, respectively. The co-expression rate of P-gp and GST-Ï€ proteins was 29.41% (15/51), of P-gp and TS proteins was 29.41%(15/51), of GST-Ï€ and TS proteins was 19.61%(10/51), of P-gp, GST-Ï€ and TS proteins was 13.73%(7/51) in cervical cancer.(2) No significant association was found between the expression of P-gp,...