The Research Of The Intervention Of Blood Pressure In Spontaneously Hypertensive Rats And The Mechanism By The Principle Of Restraining The Hyperactivity Of The Liver Yang

Objective: The research was to investigate the effects of the Principle of Restraining the Hyperactivity of the Liver Yang on blood pressure, the change of constitutional and organization form in heart and kidney and Cerebral vascular, the change of anti-oxidation serum enzyme activity, the protein expression of Angiotensin II receptors , and the electricity physiology feature of L-type calcium channels in the vascular smooth muscle cells in spontaneously hypertensive rats(SHR), to further explain the mechanism of the Principle of Restraining the Hyperactivity of the Liver Yang to the intervention of blood pressure , In the interests of Traditional Chinese Medicine on preventing and treating hypertension supply the test foundation.Methods: 12-week-old SHRs were assigned randomly into five groups: group A (treated with Tian ma gou teng decoction), group B (treated with Tian ma gou teng yin with subtraction concha haliotidis), group C(treated with Nifedipine), group D (treated with concha haliotidis), group E (treated with Normal Sodium, group control), each group consisting of nine rats. After 4-week-treatments, we measured systolic blood pressure(SBP), Sections of heart,kidney and Cerebralvascular tissue was stained with HE method , Measurement the serum enzyme activity of T-SOD(CuZn-SOD),GSH-Px,MDA by UV-spectrophotometer, Measurement the protein expression of angiotensin II type I and type II receptors in Kidney vascular were shown by labeled strept-avidin-biotin complex method of SABC, Measurement the serum free calcium concentration, Analysis the electricity physiology feature of L-type calcium channels in the vascular smooth muscle cells by patch clamp technique(PCT).Results: â‘ After 4-week-treatments, SBP was decreased in group A, There were significant differences between pre- treatment and post-treatment of group A (P<0.01). There were significant differences between group A and group E (group control) (P<0.05). There were significant differences between group C and group control after...