The Experimental Study On The Effect Of Electro-acupuncture To The Points Immunocytokin Of Insoninia Rats

Insomnia is the most common sleeping disorder characterizedby difficulty in falling asleep and sleep maintenance, aclinical common symptom which occurrence rate is very high.At present, the pathogenic mechanism of insomnia hasn't beendefined completely, and the effect of the chemotherapy isn'tgood. But experience on the treatment of insomnia by acupunctureand moxibustion of Traditional Chinese Medicine has alreadybeen used for several thousands years, the reports in recentyears showed that the curative effect on insomnia of acupunctureand moxibustion was quite satisfactory. However, the studyaiming at treatment of insomnia needs improvement, the researcharound insomnia is mostly concentrated on the clinical research,the laboratory studies especially the studies on the AdjustmentMechanism of Human Body Fluid of insomnia are comparativelyless.In order to explore the functional mechanism of insomniatreated by acupuncture, the experiment was prepared from 40Wistar rats, establishment of rat model of insomnia was adoptedby intraperitoneal injection with PCPA(para-chlorophenylalanine), using electric needle on the pointof Shenmen (HT7), Zhaohai (EI6), Dazhui (DU14) and Baihui(DU20), inquired into the mechanism of acupunctural adjustmenton sleep status of insomnia rats by evaluating the influenceof acupuncture to the contents of cerebral and serumimmunocytokine IL-1,IL-6 and TNF etc of the insomnia rats.Results:1) The result didn't show significantdifference(P＞0.05) by comparing the contents of the cerebraland serum IL-1, IL-6 and TNF-αbetween the control group andmodel group. 2) After administration of the western medicine(DIAZEPAM), the contents of cerebral and serum IL-1,IL-6 and TNF-αof insomnia rats due to PCPA were increasedsignificantly from the original level, and showed significantdifference (P＜0.05) comparing with control groups. 3) afterthe pinprick's(electric needle) treatment, the contents ofcerebral and serum IL-1, IL-6 and TNF-αof insomnia rats duedto PCPA were increased significantly from the original level,and showed very significant difference (P＜0.01) comparing withcontrol groups. 4) after the treatment, the amounts of cerebraland serum IL-1, IL-6 and TNF-αof insomnia rats dued to PCPAwere all increased significantly from the original level, butthe difference between electric needle group and westernmedicine group was significant (P＜0.05).Conclusion:1) Intraperitoneal injection with PCPA can makesleep-wakefulness cycle of normal rats disappear, and which isa quite stable establishing method of rat model of insomnia. 2)Electro-Acupuncture can increase the contents of IL-1, IL-6and TNF-αof insomnia rats in cerebrum and serum dued to PCPA,and the difference was very significant comparing with thecontrol group (P＜0.01). 3) the treatment by electric needleand western medicine, both can increase the concentrations ofcerebral and serum IL-1, IL-6 and TNF-αof insomnia rats duedto PCPA, but the effect of acupuncture was better than DIAZEPAM.4) Acupunctural adjustment on the cytokine disorders ofinsomnia rats or increasing the content of the immunocytokineis one of the mechanisms of effective treatment on insomnia byelectro—acupuncture, which will provide more objectivetheoretical foundation for the clinical treatment on insomniawith acupuncture.