The Relationship Between The Expression Of Heat Shock Protein 90,60 And 27 And Cell Damage In Peripheral Blood Of Workers Exposed To Coke Oven Emissions

Objective To explore the relationship between the expression of heat shock protein 90,60 and 27(HSP90,HSP60 and HSP27)and cell damage in peripheral blood of workers exposed to coke oven emissions.Methods 288 coke oven workers in X steel factory were divided into high dose group and low dose group on the basis of environment monitoring result and work place.There were 172 men in high dose group and 116 men in low dose group.While 38 workers unexposed occupationally carcinogenic substances were selected as the control group,who were employed in medical therapy unit nearby 2 kilometers from steel factory.We investigated their general information,history of personal and occupational exposure,and the work environment.Blood samples were collected immediately after a shift at the end of a working day from 288 coke oven workers and 38 control workers.Levels of HSP90,HSP60 and HSP27 in peripheral blood lymphocytes were measured by Western blot.LDH and MDA were measured by ELISA. Chromosomal aberrations and the degree of DNA damage in peripheral blood lymphocytes were detected by comet assay.Results There were no significant differences among those groups regarding age,sex, smoking and drinking habits(P＞0.05).Levels of HSP90(ratio)in peripheral blood lymphocytes in three groups were separately 0.24±0.32,0.12±0.30 and 0.06±0.33.They increased significantly compared with that of the control.But levels of HSP60 and HSP27 were not significantly different among those groups.Monitoring result of LDH appeared that the level of LDH in the high dose group((246.7±42.79)U/L)was higher than those in the low dose group ((205.7±33.72)U/L)and in the control group((199.7±31.78)U/L)(P＜0.05).Detectable result of MDA presented that the level of MDA in the high dose group((2.68±0.67)nmol/ml)was bigger than those in the low dose group((2.35±0.58)nmol/ml)and in the control group ((2.24±0.66)nmol/ml)(P＜0.05).Compared with the control group,there was significant difference in tail length,Olive Tail Moment of occupational exposure workers,high dose group＞low dose group＞control group(P＜0.05).The degree of DNA damage increased with the rising of exposure BaP dose(χ~2=36.411,P＜0.001;Spearman r=-0.345,P＜0.001).There is a dose-effect relationship between Olive and exposed dose of PAHs.(β=-0.117,P=0.030)Conclusion 1.Result of comet assay showed that Coke oven emission could result in genetic damage.2.The increase of HSP90 level may be involved in protection of DNA from genotoxic damage induced by carcinogen.3.LDH and MDA in peripheral blood and HSP90 in peripheral blood of coke oven workers could offer scientific evidence for the study of an exposure biomarker to evaluate PAHs contact level.