| Objective : Testicular torsion with a high incidence in young adolescence is a clinical emergency and need to be re-ducted early to save the testes. However, after dertor-sion ,functional testicular atrophy in varying degrees ,even lower germ has become need to be resolved. Recent study of spermatogenesis found that testicular reperfusion injury is caused by the production of ROS, including hydrogen peroxide (H2O2), hydroxyl (-OH) and superoxide radicals Mission (O2-). They cause mitochondrial membrane lipid peroxidation , un-dermine the integrity of the membrane and increase membrane permeability. Then lead to cell damage and apoptosis in the testis. [1] Testicular torsion not only leads to the direct damage of the affected testis, but also leads to a sympathetic contralateral testicular injury[2]. The mechanism of the contralateral testicular injury may be related to contralateral testicular ische-mia-reperfusion, immune factors, neurohumoral factors and the blood-testis barrier. Through this experimental animal model of testicular torsion/detorsion. We aimed to investigate the protec-tive effect of testicular torsion/detorsion and the product re-lated to biochemical changes and the changes in the germ cell apoptosis. For the clinical patients with testicular torsion of testicular damage and reducing spermatogenesis protection ,the experiment provided experimental evidence and theoretical basis .Methods : 40 adult(6-8W) male Wistar rats were randomly divided into testicular torsion sham control group (group A), testicular torsion/detorsion group (Group B), testicular torsion detorsion and single astragalus injection treatment group (group C), testicular torsion detorsion and many astragalus injection treatment group (Group D),10 in each group. The unilateral testicular torsion /detorsion model was estabilished according to Turner method [3] : 2% pentobarbital sodium (5mg/Kg) intrap-eritoneal injection of anesthesia, small abdominal incision, the left testis was free,then cut off testicular lead and isolate epidi-dymal head to the surrounding fascia. Group A : left scrotal incision, free exposed testes, arbitrarily moved model, but not reversed,then sutured the scrotum. Group B : left testicle was rotated of 720°colckwisely around spermatic cord, sutured of tunica albuginea to prevent spontaneous detorsion ,then sutured scrotum. After detorsion for 6 hours the left testes were reversed and fixed by operation. Group C : We made in the same way as Group B. In the 30 minutesbefore detorsion (that was 5.5h after the torsion) ,astragalus injection (3g/Kg) was intraperitoneal injected. Group D : The ways were the same as the ways of Group B to reverse .In addition to the 30 minutes before(that was 5.5h after the torsion) , also on the first postoperative day, two days, three days astragalus injection (1g/Kg) were intrap-eritoneal astragalus injection. In the seven days after testicular torsion/detorsion, bilateral testes were obtained for paraf-fin-embedded sections and homogenate to be examined.Paraffin-embedded sections of testicular tissue were measured in TdT-mediated d-UTP nick end labeling (TUNEL) method to investigate germ cell apoptosis. Each selected five sections vision optical microscope (×200), counted 100 cells, and percentage of positive cells apoptosis has been obtained. Determination of the chemical testicular tissue is to investigate superoxide dismutase (SOD) and malondialdehyde (MDA). These results are used, SPSS12.0 statistical data software was used for statistical analysis. Homogeneity test methods used single-factor analysis of variance.For each of these analy-ses,two-tailed probability values of <0.05 are designed as sig-nificant.Results :The results of bilateral testes show : 1 Single as-tragalus injection treatment group was compared with testicular torsion/detorsion group : SOD levels significantly increased and the difference was significant (P<0.05). Content of MDA were reduced drastically and the difference was significant (P<0.05). Apoptosis index was significantly reduced and the difference was significant (P<0.05).2 Many astragalus injection treatment group was compared with testicular torsion/detorsion group : SOD levels were signifi-cantly higher and the difference was significant (P<0.05). Con- tent of MDA was significantly reduced drastically and the dif-ference was significant (P<0.05). Apoptosis index was signifi-cantly lower and the difference was significant (P<0.05).3 Many astragalus injection treatment group was compared with single astragalus injection treatment group group : SOD levels were significantly higher and the difference was significant (P <0.05). Content of MDA was significantly reduced drastically and the difference was significant (P<0.05). Apoptosis index was significantly lower and the difference was significant (P <0.05).4 Testicular torsion/detorsion group, single astragalus injection treatment group and many astragalus injection treatment group were compared with sham treatment group each other:SOD levels were significantly lower than the control group ; the con-tent of MDA was significantly higher; apoptosis index was sig-nificantly lower.The difference was significant each other(P <0.05).Conclusion : 1 Astragalus injection could reduce the bilat-eral germ cell reduction and can protect the testicular germ cell after testicular torsion /detorsion .2 Astragalus injection may improve antioxidant enzyme activity and reduce the production of oxygen-derived free radicals so as to reduce rat testicular ischemia-reperfusion injury after testicu-lar torsion/detorsion.
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