Protective Effect And Mechanism Of Xinmaitong Serum On Myocardial Cell Apoptosis In Oxidative Stress Induced By Peroxide

OBJECTIVE:Myocardial ischemia-reperfusion injury is the difficult problem in the treatment of ischemic heart disease. One of the main mechanisms is the large amount of oxygen free radical which leads to structural damage and functional or metabolic disorder. Currently, great attention has been paid on Traditional Chinese Medicine for its protective effect against ischemia-reperfusion injury. In this research, we investigate the effect and the possible mechanism of Xinmaitong serum protecting myocardial cell against apoptosis induced by peroxide.METHODS:Peroxide was used to construct oxidative stress model. Myocardial cell apoptosis was induced by 400μmol/H₂O₂. There were 5 groups in our research: normal control group; two module groups : pretreated myocardial cells with H₂O₂ for 1h and 6h; Xinmaitong serum group: pretreated myocardial cells with H₂O₂ for 1h and 6h, then added Xinmaitong serum for cultivation for 24h. Observed the morphological changes of myocardial cell under inverted phase contrast microscope; detected cell viability by MTT chromatometry; myocardial cell apoptosis was detected by TUNEL; the expression and distribution of NF-κB, Caspase-3 and HSP70 was detected by immunocytochemistry.RESULETS:When cardiocytes were treated with H₂O₂ for 1h, the statistical analysis showed that there was no statistical difference in cell viability, apoptosis index, Nuclear translocation rate of NF-κB, the activation of Caspase-3 and the expression of HSP70 in model group and Xinmaitong group compared with normal group. When cardiocytes were treated with H₂O₂ for 6h, cell viability in model group decreased apearently, while the apoptosis index, Nuclear translocation rate of NF-κB and the activation of Caspase-3 was significantly higher than the normal group, the expression of HSP70 increased lightly. Compared with modle group, the cell viability in Xinmaitong group was significantly improved, the expression of HSP70 was significantly higher, while the apoptosis index, Nuclear translocation rate of NF-κB and the activation of Caspase-3 was significantly lower than the model group.CONCLUSIONS:(1)Peroxide damaged neonate rat cardiocytes in a way of time dependence.(2) Xinmaitong serum could protect cardiocytes by reducing the cell viability and inhibiting cell apoptosis that induced by peroxide.(3) The activation of NF-κB and Caspase-3 played an important role in caridoctye apoptosis, but HSP70 showed a protective effect.(4) Xinmaitong inhibited cardiocytes apoptosis possibly by preventing the activation of NF-κB and Caspase-3 while promoting the expression of HSP70.