Comparison To The Myocardial Protective Effects And Toxic Reactions Of Sodium Hydrosulfide At Different Doses

Objective(1)To object the myocardial protective effects of NaHS treatment with intraperitoneal injection at different doses.(2)To investigate the toxic reaction of NaHS solution injected intraperitoneally at different doses to the important vital organs,such as heart,liver,kidney and lung.Methods(1)35 Wistar rats were included and divided averagely into five groups named sham operation group,myocardial ischemia/reperfusion(I/R)model group,NariSⅠgroup,NaHSⅡgroup and NaHSⅢgroup at random,each group included 7 rats;The rats in three NaHS treatment groups were injected intraperitoneally(i.p)with the NaHS at doses of 1 mg/kg,3 mg/kg and 5 mg/kg respectively,while the same volume of physiological salt solution injected intraperitoneally in Sham and I/R model groups.After 15 minutes of intraperitoneal injection,all rats were subjected to opening chest and the left anterior descending coronary arteries(LAD) were ligated for 30 minutes and unclamped for 60 minutes except the rats with only thread-drawing in Sham group.Electrocardiogram and hemodynamics of all rats was measured. such as left ventricular systolic pressure(LVSP),left ventricular end diastolic pressure(LVEDP), maximal rate of rise of left ventricular pressure(±dp/dt max).Ischemic zone size and infarct size and left ventricle were also weighed.(2)28 Wistar rats were included and divided averagely into four groups named control group, NaHSⅠgroup,NaHSⅡgroup and NaHSⅢgroup at random,each group included 7 rats; The rats in three NaHS treatment groups were injected intraperitoneally(i.p)with the NaHs at doses of 1 mg/kg,3 mg/kg and 5 mg/kg respectively,After 105 minutes of intraperitoneal injection.all rats were killed and the hearts,lungs,kidneys,and livers of every rat were conserved with formalin liquid.Then sections stained with hematoxylin-eosin were examined by light microscopy for evidence of injury of all organs.Results(1)Ischemic zone size was not different among the groups except sham group(P＞0.05). Infarct size was significantly lower in NaHSⅠgroup andⅡgroup than I/R group(24.27± 5.94 versus 37.32±6.2,P＜0.01 and 30.56±5.48 versus 37.32±6.2,P＜0.01,respectively),and there was no difference between the NaHSⅢgroup and I/R group(36.85±7.01 vs 37.32±6.2,P＞0.05).(2)The levels of LVSP and±dp/dt max in all groups took on a descending trend,especially in the I/R group and the NaHsⅢgroup;The levels of LVEDP assumed a ascending trend in all groups,and those in the I/R group and the NaHSⅡgroup were most obvious with those in the NaHSⅢgroup.Compared with the sham group,the levels of +dp/dt max in the I/R group and the NaHSⅢgroup were overtly decreased(12.6%and 12.2%respectively,P＜0.01),the levels of-dp/dt max deceased respectively by 13.8%and 13.2%(P＜0.01),and the levels of LVSP deceased respectively by 11.3%and 11.7%(P＜0.01);The levels of±dp/dt max and LVSP were higher significantly in the NaHSⅠgroup and the NaHSⅡgroup than those in the I/R group and the NaHSⅢgroup(P＜0.05),and the levels of LVEDP in the NarisⅠgroup deceased by 14.2% compared with those in the I/R group(P＜0.01).Compared with the NaHSⅠgroup,the levels of LVEDP increased respectively by 13.4%and 14.5%in the NaHSⅡgroup and the NaHSⅢgroup(P＜0.01)(3)Results from morphometric analysis demonstrated there were prominent edema, infiltration of neutrophils,and intrapulmonary hemorrhage in the lungs of animals in the NaHSⅡandⅢgroups when compared with sham group.In the NaHSⅠgroup,there was significantly alleviated edema,infiltration of neutrophils,and intrapulmonary hemorrhage when compared with the NaHSⅡandⅢgroups(P＜0.05).But when the hearts,kidneys and livers were calculated,there was no difference among every group.Conclusion(1)NaHSⅠandⅡgroups had myocardial preservative effects,but NaHSⅢgroup did not so.(2)Various organs were injured by NaHS injected intraperitoneally,and the lung was one of the main target organs of toxic action of hydrogen sulfide.This damage mechanism of hydrogen sulfide is possibly related with high liposolubility.(3)From NaHSⅡgroup,we concluded that NaHS with intraperitoneal injection damaged severely the lungs of rats while induced myocardial preservative effects.