Experimental Study On The Change Of Rabbit's ICAM-1 Content After CNV And Penetrating Keratoplasty

Objective: The levels of sICAM-1 in aqueous humor and serum were determined different time after CNV and penetrating keratoplasty surgery. To investigate the role of ICAM-1 in the graft rejection of rabbit CNV and prediction of corneal graft rejection .Methods: Suture was used to make corneal neovascularization model on 20 new zealand white rabbits. Then making penetrating keratoplasty model four groups;A: normal group , B:autograft group ,C:heterograft group D:CNV rabbit models of penetrating keratoplasty. Neovascularization, graft mean survival times and rejection index were determined. The levels of sICAM-1 in serum were dynamically determined by enzyme-linked immunosobent assay(ELISA) on 3,7,14,21 and 28 days or in aqueous humor time of rejection after surgery. The HE(Hematoxylin eosin)and immunohistochemical technique were performed on corneal.Results: Neovascularization and on central corneal 16.4±1.8d, graft mean survival times12.4±1.3d, The mean ICAM-1 levels in aqueous humor and serum in the control animals that did not undergo surgery were16.55±3.63 ng·L-1, 95.15±6.26 ng·L-1, Levels increased in the early stages postoperatively in all surgical groups. Subsequently,levels decreased in the autograft group and heterograft group,while high levels were sustained in the CNV group. Levels for the CNV group continued to increase and were 53.87±19.23 ng·L-1,378.78±30.58 ng·L-1 when compared to those of the other group (P<0.01). Correlation analysis showed the change in sICAM-1 had appositive correlation between the aqueous humor and serum(r=0.999,P<0.05).The CNV groups, there were inflammatory cells had spread into the corneal when rejection occurred. There were even more lymphocytes and macrophagocytes in the CNV group. we could also find ICAM-1 positive cells in the graft rejection group.Conclusion: Intercellular adhesion Molecule-1 (ICAM-1) plays a critical role in the pathogenesis of initiating and maintaining corneal graft rejection. Sequential determination of serum sICAM-1 after operation may be of value in the prediction and diagnosis of acute rejection .